Lavergne D, de Villiers E M
Division of Tumor Virus Characterization, Applied Tumor Virology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Int J Cancer. 1999 Mar 1;80(5):681-4. doi: 10.1002/(sici)1097-0215(19990301)80:5<681::aid-ijc8>3.0.co;2-a.
Human papillomavirus (HPV) has been implicated as a possible etiological factor in the development of squamous-cell carcinoma of the esophagus. Published data demonstrating HPV DNA in these lesions have been conflicting, varying between failure to detect HPV DNA to detection of up to 60% to 70% of the biopsies harboring HPV DNA, mainly HPV 16 and HPV 18. We have analyzed esophageal carcinoma samples from 2 high-risk areas, China and South Africa, using a degenerate PCR approach. All amplified products were cloned and sequenced. A broad spectrum of HPV types was demonstrated in 10/29 samples from China and 9/34 samples from South Africa. HPV types detected included mucosal types HPV 6, 18, 51, 52 and 57; cutaneous types HPV 9, 20, 24 and 25; and the putative new HPV types DL231, DL428 and DL436, with HPV 6/51, 6/57, 20/9 and 20/DL231 occurring as double infections. HPV 6 predominated (4 samples) in 11 esophageal papillomas originating from patients in Europe and tested for HPV DNA. Other HPV types present included HPV 20, DL284 and DL436. Another putative new HPV type, DL416, was identified in a dysplastic lesion of the esophagus.
人乳头瘤病毒（HPV）被认为可能是食管鳞状细胞癌发生的一个病因。已发表的数据表明，这些病变中HPV DNA的检测结果相互矛盾，从未能检测到HPV DNA到检测到高达60%至70%的活检样本中含有HPV DNA，主要是HPV 16和HPV 18。我们使用简并PCR方法分析了来自中国和南非这两个高危地区的食管癌样本。所有扩增产物均进行了克隆和测序。在中国的29个样本中有10个、南非的34个样本中有9个检测到了广泛的HPV类型。检测到的HPV类型包括黏膜型HPV 6、18、51、52和57；皮肤型HPV 9、20、24和25；以及推定的新HPV类型DL231、DL428和DL436，其中HPV 6/51、6/57、20/9和20/DL231为双重感染。在来自欧洲患者的11个食管乳头状瘤中检测HPV DNA，其中HPV 6占主导（4个样本）。其他存在的HPV类型包括HPV 20、DL284和DL436。在食管发育异常病变中鉴定出另一种推定的新HPV类型DL416。
