兔网织红细胞合成的膜蛋白。
Membrane proteins synthesized by rabbit reticulocytes.
作者信息
Lodish H F, Small B
出版信息
J Cell Biol. 1975 Apr;65(1):51-64. doi: 10.1083/jcb.65.1.51.
Abstract
Intact rabbit reticulocyte cells synthesize two predominant species of polypeptides which are components of the cell plasma membrane. Previous work (Lodish, H. F. 1973. Proc. Natl. Acad. Sci. U. S. A. 70:1526-1530.) showed that these proteins were synthesized by polyribosomes not attached to membranes. We show here that both polypeptides are confined to the cytoplasmic surface of the cell membrane. These studies utilized iodination of whole cells and of membranes with lactoperoxidase, and digestion of whole cells and membranes with chymotrypsin, One of these proteins is synthesized as a precursor, and about 20-40 amino acids are removed after it is incorporated into the membrane, We discuss the probable sites of synthesis of these and other classes of membrane proteins.
摘要
完整的兔网织红细胞能合成两种主要的多肽，它们是细胞质膜的组成成分。先前的研究工作（洛迪什，H. F. 1973年。美国国家科学院院刊70:1526 - 1530。）表明，这些蛋白质是由不附着于膜的多核糖体合成的。我们在此表明，这两种多肽都局限于细胞膜的细胞质表面。这些研究利用了乳过氧化物酶对完整细胞和膜进行碘化，以及用胰凝乳蛋白酶对完整细胞和膜进行消化，其中一种蛋白质是以前体形式合成的，在它被整合到膜中后约20 - 40个氨基酸被去除，我们讨论了这些以及其他类膜蛋白可能的合成位点。
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