Obinata M, Ikawa Y
Nucleic Acids Res. 1980 Sep 25;8(18):4271-82. doi: 10.1093/nar/8.18.4271.
The change in the poly A(+) mRNA population during erythrodifferentiation was analyzed by cDNA-RNA hybridization. Poly A(+) RNA was isolated from spleen erythroblasts. When mice became anemic, the amount of globin mRNA increased to 50% of the total poly A(+) mRNA. cDNA from anemic spleen erythroblasts that did not contain globin mRNA sequences was cross-hybridized with mRNAs from mouse reticulocytes and cultured Friend leukemia (FL) cells. Only half the spleen cDNA hybridized with reticulocyte mRNA, whereas most of it hybridized with mRNA from FL cells. The results suggest that decrease in the complexity of the message population and increase in the concentration of globin mRNA are important in erythrodifferentiation.
通过cDNA-RNA杂交分析了红细胞分化过程中多聚腺苷酸(Poly A)(+) mRNA群体的变化。从脾脏成红细胞中分离出多聚腺苷酸(Poly A)(+) RNA。当小鼠出现贫血时,珠蛋白mRNA的量增加至总多聚腺苷酸(Poly A)(+) mRNA的50%。来自不含珠蛋白mRNA序列的贫血脾脏成红细胞的cDNA与来自小鼠网织红细胞和培养的弗氏白血病(FL)细胞的mRNA进行交叉杂交。只有一半的脾脏cDNA与网织红细胞mRNA杂交,而大部分与FL细胞的mRNA杂交。结果表明,信息群体复杂性的降低和珠蛋白mRNA浓度的增加在红细胞分化中很重要。