Hayashi F, Okada M, Zhong X, Miura K
Department of Pediatric Dentistry, Hiroshima University Faculty of Dentistry, Hiroshima, Japan.
Microbiol Immunol. 2001;45(1):17-22. doi: 10.1111/j.1348-0421.2001.tb01269.x.
The purpose of this study was to detect the presence of Capnocytophaga sputigena, C. ochracea, and C. gingivalis in plaque samples from the toothbrushes of 122 children, using a polymerase chain reaction (PCR) method. The subjects were 25, 85, and 12 children with healthy gingiva, gingivitis, and periodontitis, respectively, ranging in age from 2-12 years old. Plaque samples were collected from all erupted tooth sites using a sterile toothbrush. The mean amount of DNA recovered from the samples was approximately 19.3 microg, which was deemed sufficient for performing a PCR-based survey. C. sputigena prevalence in healthy, gingivitis, and periodontitis subjects was 48.0%, 36.5% and 25.0%, respectively, that for C. ochracea was 100%, 89.4%, and 50.0%, respectively, and that for C. gingivalis was 96.0%, 84.7%, and 75.0%, respectively. The lowest age of positive subjects was approximately 2 years. Our results showed that C. sputigena was moderately prevalent, whereas C. ochracea and C. gingivalis were commonly detected in the oral cavities of the tested children, suggesting that all of these species become established in the early years.
本研究旨在采用聚合酶链反应（PCR）方法，检测122名儿童牙刷菌斑样本中具核梭杆菌、赭色梭杆菌和牙龈梭杆菌的存在情况。研究对象分别为25名、85名和12名牙龈健康、患牙龈炎和牙周炎的儿童，年龄在2至12岁之间。使用无菌牙刷从所有已萌出牙齿部位采集菌斑样本。从样本中回收的DNA平均量约为19.3微克，被认为足以进行基于PCR的调查。具核梭杆菌在健康、牙龈炎和牙周炎受试者中的患病率分别为48.0%、36.5%和25.0%，赭色梭杆菌的患病率分别为100%、89.4%和50.0%，牙龈梭杆菌的患病率分别为96.0%、84.7%和75.0%。阳性受试者的最低年龄约为2岁。我们的结果表明，具核梭杆菌患病率中等，而赭色梭杆菌和牙龈梭杆菌在受试儿童口腔中普遍检测到，这表明所有这些菌种在儿童早期就已定植。
