Shigeishi Hideo, Hamada Natsuki, Kaneyasu Yoshino, Niitani Yoshie, Takemoto Toshinobu, Ohta Kouji
Department of Public Oral Health, Program of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Department of Oral Health Management, Program of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Biomed Rep. 2024 Apr 30;20(6):99. doi: 10.3892/br.2024.1787. eCollection 2024 Jun.
species are commonly found in human oral microbiome. The aim of the present study was to understand the association of the prevalence of oral species with oral hygiene and periodontal inflammation. A total of 136 patients (median age 72 years) who visited the Hiroshima University Hospital (Hiroshima, Japan) between April 2021 and June 2023 were enrolled. Swab samples were obtained from the tongue surface. DNA from species ( and ) was detected by real-time PCR analysis. Dental plaque accumulation was observed to assess the oral hygiene condition of participants. Additionally, clinical periodontal inflammation was assessed with periodontal inflamed surface area (PISA) scores. Clinical confounding factors such as age, sex, lifestyle-related disease, remaining teeth and denture wearing between species-positive and -negative groups were adjusted with a propensity score matching method. Mann-Whitney U and χ or Fisher's exact test were employed for statistical analysis. The prevalence rate was 67.6% for oral and 83.1% for . positive participants showed significantly higher plaque control record scores (an indicator of dental plaque accumulation) than negative participants (P=0.03). Additionally, / dual-positive participants exhibited significantly higher plaque control record and PISA scores than non-dual-positive participants (P=0.01 and P=0.04, respectively). Propensity score matching was conducted in the / dual-positive group and the non-dual-positive group for adjustment of clinical factors, resulting in 51 matched patient pairs. / dual-positive participants had significantly higher plaque control record scores than non-dual-positive participants (P=0.02). The present results suggest that the prevalence of both oral and is associated with poor oral hygiene in middle-aged and older people.
某些物种常见于人类口腔微生物群中。本研究的目的是了解口腔中这些物种的流行率与口腔卫生和牙周炎症之间的关联。共有136名患者（中位年龄72岁）纳入研究，这些患者于2021年4月至2023年6月期间就诊于广岛大学医院（日本广岛）。从舌面获取拭子样本。通过实时PCR分析检测某些物种（和）的DNA。观察牙菌斑堆积情况以评估参与者的口腔卫生状况。此外，用牙周炎症表面积（PISA）评分评估临床牙周炎症。采用倾向得分匹配法对某些物种阳性组和阴性组之间的年龄、性别、生活方式相关疾病、剩余牙齿和佩戴假牙等临床混杂因素进行调整。采用Mann-Whitney U检验和χ²检验或Fisher精确检验进行统计分析。口腔中该物种的流行率为67.6%，另一物种为83.1%。阳性参与者的菌斑控制记录评分（牙菌斑堆积的指标）显著高于阴性参与者（P = 0.03）。此外，/双阳性参与者的菌斑控制记录和PISA评分显著高于非双阳性参与者（分别为P = 0.01和P = 0.04）。在/双阳性组和非双阳性组中进行倾向得分匹配以调整临床因素，得到51对匹配的患者对。/双阳性参与者的菌斑控制记录评分显著高于非双阳性参与者（P = 0.02）。目前的结果表明，口腔中这两种物种的流行率均与中老年人口腔卫生不良有关。
