Idate Ulka, Bhat Kishore, Kulkarni R D, Kotrashetti Vijayalakshmi, Kugaji Manohar, Kumbar Vijay
Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India.
Department of Microbiology, SDM Medical College, Dharwad, Karnataka, India.
J Oral Maxillofac Pathol. 2022 Apr-Jun;26(2):287. doi: 10.4103/jomfp.jomfp_172_21. Epub 2022 Jun 28.
are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a few studies attempted to identify all the seven species of phenotypically and genotypically in healthy individuals and patients with chronic periodontitis. Studies to determine the prevalence of in subgingival plaque samples from healthy individuals, chronic gingivitis and periodontitis among Indian population are lacking.
The aim of this study was to identify and compare the presence of species phenotypically through microbial culture and biochemical tests and genotypically through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in subgingival plaque of healthy individuals and patients with chronic gingivitis and chronic periodontitis.
A total of 300 subjects, 100 each with gingivitis, periodontitis and periodontally healthy gingiva subjected, were included. Subgingival plaque was collected and was cultured for phenotypic identification (microbial culture and biochemical test), and for genotypic identification, DNA extraction was done and PCR-RFLP analysis was performed to identify the genus and also to identify different species of .
Of 300 individuals, species were identified from 237 (79%) individuals by PCR and 82 (27.33%) by culture. The prevalence of was found to be higher with both the methods followed by and . , and were isolated only by culture with very low prevalence that is 1.33%, 1.33% and 0.66%, respectively. We could not get any isolate of by any of the two methods.
species could be found in gingival sulci as well as periodontal pockets and can be detected by culture and PCR-RFLP. However, higher prevalence of these species in healthy compared to disease requires further analysis to determine their role in healthy and diseased periodontium.
[某种细菌名称]是兼性厌氧革兰氏阴性杆菌，被认为是各种口外感染的机会性病原体。仅有少数研究试图在健康个体和慢性牙周炎患者中从表型和基因型上鉴定出该细菌的所有七个种。目前缺乏关于印度人群中健康个体、慢性牙龈炎和牙周炎患者龈下菌斑样本中该细菌流行情况的研究。
本研究的目的是通过微生物培养和生化试验从表型上以及通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）从基因型上鉴定和比较健康个体、慢性牙龈炎患者和慢性牙周炎患者龈下菌斑中该细菌种的存在情况。
共纳入300名受试者，其中牙龈炎、牙周炎和牙周健康牙龈各100名。收集龈下菌斑，进行培养以进行表型鉴定（微生物培养和生化试验），对于基因型鉴定，进行DNA提取并进行PCR-RFLP分析以鉴定该细菌属以及该细菌的不同种。
在300名个体中，通过PCR从237名（79%）个体中鉴定出该细菌种，通过培养从82名（27.33%）个体中鉴定出。两种方法均发现该细菌的流行率较高，其次是[其他细菌名称1]和[其他细菌名称2]。[其他细菌名称3]、[其他细菌名称4]和[其他细菌名称5]仅通过培养分离出，流行率极低，分别为1.33%、1.33%和0.66%。通过两种方法中的任何一种我们都未分离出[另一种细菌名称]。
该细菌种可在牙龈沟以及牙周袋中发现，可通过培养和PCR-RFLP检测到。然而，与疾病状态相比，这些种在健康状态下的流行率较高，需要进一步分析以确定它们在健康和患病牙周组织中的作用。
