Molecular identification of species from the oral cavity of patients with chronic periodontitis and healthy individuals.

INTRODUCTION

species are recognized as part of human oral microbiota and implicated as periodontal pathogens associated with various periodontal diseases. The three original species - and were initially isolated from periodontitis in adults, but subsequent studies demonstrated their presence also at periodontally healthy sites in both children and adults. Their association with periodontal disease is a matter of controversy. Considering the differing virulence features of the respective isolate, it is crucial to identify these isolates to species level.

AIMS

The aim of this study is to investigate the prevalence of species by polymerase chain reaction (PCR) through restriction fragment length polymorphism in healthy individuals and patients with periodontal disease.

MATERIAL AND METHODS

The study included a total of 300 individuals, 100 each with Gingivitis, Chronic periodontitis, and Healthy individuals. The plaque samples were collected using sterile curette in reduced transport fluid. DNA extraction was carried out for PCR analysis.

RESULTS

Of 300 individuals, species were identified from 237 (79%) participants in all groups. The prevalence was statistically analyzed using Chi-square test. The prevalence was more in males in gingivitis and healthy individuals (42% and 49% respectively), and females in periodontitis (40%). was observed in a higher proportion (36.33%), followed by (32.66%) and (10%). They were identified more in the age group of 30-40 years in gingivitis and periodontitis, (30 and 21 individuals, respectively) and 39 individuals in 18-29 years in healthy individuals. They were present in 87% in healthy individuals, 77% in gingivitis and 73% in periodontitis.

CONCLUSION

species are commonly present in healthy individuals and may be associated with periodontal disease. There is a need for further study to know the prevalence of other species of in health and disease.