Santucci A, Trabalzini L, Bovalini L, Ferro E, Neri P, Martelli P
Dipartimento di Biologia Molecolare, Università degli Studi di Siena, Italy.
Electrophoresis. 2000 Nov;21(17):3717-23. doi: 10.1002/1522-2683(200011)21:17<3717::AID-ELPS3717>3.0.CO;2-4.
We recently studied the protein composition of a Saccharomyces cerevisiae wine yeast strain (K310) of enological interest. About 2,500 spots of 8-250 kDa observed molecular mass were resolved by two-dimensional gel electrophoresis. Experimental molecular masses and isoelectric points were calculated for most of them. Twenty-seven proteins were subjected to Edman microsequencing. N-terminal sequences of 12/27 proteins were determined, whereas internal sequences of 6/27 proteins were obtained following in situ proteolysis. Comparison between the experimental data and those reported in the SWISS-PROT database revealed some differences between genotypic and phenotypic sequences. These are indicative of the changes a protein can undergo with respect to the primary structure coded by the genomic DNA. Our results highlight the need to complement genomic analysis with detailed proteomics in order to refine the vast amount of information provided by DNA sequencing and to find an exact correlation between genome and proteome.
我们最近研究了一株具有酿酒学意义的酿酒酵母葡萄酒酵母菌株（K310）的蛋白质组成。通过二维凝胶电泳分离出了约2500个观察到的分子量在8 - 250 kDa之间的斑点。计算了其中大多数蛋白质的实验分子量和等电点。对27种蛋白质进行了埃德曼微量测序。确定了27种蛋白质中12种的N端序列，而在原位蛋白酶解后获得了27种蛋白质中6种的内部序列。实验数据与SWISS - PROT数据库中报告的数据之间的比较揭示了基因型和表型序列之间的一些差异。这些差异表明蛋白质相对于基因组DNA编码的一级结构可能发生的变化。我们的结果强调了用详细的蛋白质组学补充基因组分析的必要性，以便完善DNA测序提供的大量信息，并找到基因组与蛋白质组之间的确切关联。
