Boucherie H, Sagliocco F, Joubert R, Maillet I, Labarre J, Perrot M
Institut de Biochimie et Génétique Cellularies, UPR CNRS 9026, Bordeaux, France.
Electrophoresis. 1996 Nov;17(11):1683-99. doi: 10.1002/elps.1150171106.
With the systematic sequencing of the yeast genome, yeast biology has entered a new era where novel challenges have to be faced. One challenge is the identification of the function of the several hundred novel genes discovered by genome sequencing. Another is to understand how all yeast genes act in concert to ensure and maintain cell organization. Two-dimensional (2-D) gel electrophoresis is the technique of choice to take up these challenges because it provides the opportunity of obtaining an overall view of genome expression. In prospect of these studies we have undertaken the construction of a yeast 2-D gel protein database that contains information on polypeptides of the yeast protein map. In this paper we report the information presently contained in this database. The reported information includes the identification of 250 protein spots and the characterization of polypeptides corresponding to N-terminal acetylated proteins, mitochondrial proteins, glucose-repressed proteins, heat shock induced proteins and proteins encoded by intron-containing genes. In all, 600 spots are annotated. These data can be accessed on the Yeast Protein Map server through the World Wide Web network.
随着酵母基因组的系统测序，酵母生物学进入了一个必须面对新挑战的新时代。一个挑战是确定通过基因组测序发现的数百个新基因的功能。另一个挑战是了解所有酵母基因如何协同作用以确保和维持细胞组织。二维（2-D）凝胶电泳是应对这些挑战的首选技术，因为它提供了获得基因组表达全景的机会。鉴于这些研究，我们已着手构建一个酵母二维凝胶蛋白质数据库，该数据库包含酵母蛋白质图谱中多肽的信息。在本文中，我们报告了该数据库目前包含的信息。报告的信息包括250个蛋白质斑点的鉴定以及与N端乙酰化蛋白、线粒体蛋白、葡萄糖抑制蛋白、热休克诱导蛋白和含内含子基因编码的蛋白相对应的多肽的表征。总共注释了600个斑点。这些数据可通过万维网在酵母蛋白质图谱服务器上获取。
