Robredo B, Singh K V, Torres C, Murray B E
Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
Microb Drug Resist. 2000 Winter;6(4):305-11. doi: 10.1089/mdr.2000.6.305.
The present study was performed to determine if any of the 45 vanA-containing Enterococcus faecium or 18 vanA-containing E. hirae strains were shared by chickens (32 E. faecium/l7 E. hirae) and humans (13 E. faecium/1 E. hirae) using pulsed field gel electrophoresis (PFGE) and to study quinupristin-dalfopristin (Q-D) resistance. Seven of the 45 E. faecium isolates (from 2 outpatients and from 5 poultry products) were resistant to Q-D (MIC > or = 16 microg/ml); one strain was shown to have satA by PCR and sequencing and, in the other six isolates, the recently described satG gene was demonstrated. Six different PFGE patterns were detected among the 7 Q-D E. faecium-resistant isolates. None of the E. hirae isolates showed Q-D resistance. Among the 45 vanA -containing E. faecium strains, 25 unrelated clones were found by PFGE with highly diverse patterns and an indistinguishable PFGE pattern was observed in vanA-containing E. faecium strains from two humans and two poultry products. A single PFGE pattern was detected in 17 of 18 vanA-containing E. hirae isolates, obtained from one human and 16 chicken samples. Based on the presence of indistinguishable PFGE patterns among VR E. faecium and E. hirae from humans and chickens, we conclude that horizontal transfer of these strains could occur between both groups.
本研究旨在通过脉冲场凝胶电泳（PFGE）确定45株含vanA的粪肠球菌或18株含vanA的希氏肠球菌中是否有菌株同时存在于鸡（32株粪肠球菌/17株希氏肠球菌）和人类（13株粪肠球菌/1株希氏肠球菌）中，并研究奎奴普丁-达福普汀（Q-D）耐药性。45株粪肠球菌分离株中的7株（来自2名门诊患者和5种禽肉产品）对Q-D耐药（MIC≥16μg/ml）；通过PCR和测序显示其中1株有satA基因，另外6株分离株中检测到最近描述的satG基因。在7株对Q-D耐药的粪肠球菌分离株中检测到6种不同的PFGE图谱。希氏肠球菌分离株均未显示对Q-D耐药。在45株含vanA的粪肠球菌菌株中，通过PFGE发现25个不相关的克隆，其图谱高度多样，并且在来自两名人类和两种禽肉产品的含vanA的粪肠球菌菌株中观察到无法区分的PFGE图谱。在从1名人类和16份鸡样本中获得的18株含vanA的希氏肠球菌分离株中，检测到单一的PFGE图谱。基于来自人类和鸡的耐万古霉素粪肠球菌和希氏肠球菌中存在无法区分的PFGE图谱，我们得出结论，这些菌株可能在两组之间发生水平转移。
