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药用植物L.野生种群的遗传多样性评估

Evaluation of genetic diversity in wild populations of L., a medicinal plant.

作者信息

El-Bakatoushi Ranya, Ahmed Dalia Gamil Aseel

机构信息

Biological and Geological Sciences Department, Faculty of Education, University of Alexandria, Egypt.

Plant Protection and Biomolecular Diagnosis Department, Arid Land Cultivation and Research Institute (ALCRI) Department, City for Scientific Research and Technological Applications, Egypt.

出版信息

J Genet Eng Biotechnol. 2018 Jun;16(1):143-151. doi: 10.1016/j.jgeb.2017.11.007. Epub 2017 Nov 26.

DOI:10.1016/j.jgeb.2017.11.007
PMID:30647716
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6296603/
Abstract

L. is a perennial herbaceous plant and can be a future drug due to its wide medicinal purposes. Despite its economic importance, the molecular genetics of have not yet been studied in detail. Genetic diversity of 12 genotypes were investigated by using Inter-Simple Sequence Repeats (ISSR), PCR-RFLP of rDNA-ITS, PCR-SSCP of rDNA-ITS and Simple Sequence Repeat (SSR) markers. The level of polymorphism revealed by ITS-SSCP is the lowest, followed by ITS-RFLP then ISSR and the highest polymorphism level was reported for SSR marker. The AMOVA analysis implied that most of the variation occurred within the Populations. A value of inbreeding coefficient estimated by the three co-dominant markers was nearly equal and offer an indication of the partial out-crossing reproductive system of Principal Coordinate Analysis (PCOA) plot revealed a clear pattern of clustering based on the locations of collected plants which coincide with the isolation by distance. The study revealed that ITS-SSCP and ISSR markers respectively were more informative than the other used markers in the assessment of genetic diversity of . The results reflect the great diversity of and data obtained from this study can be used for future collecting missions.

摘要

L.是一种多年生草本植物，因其广泛的药用价值，有望成为未来的药物。尽管其具有经济重要性，但其分子遗传学尚未得到详细研究。利用简单序列重复区间（ISSR）、核糖体DNA内转录间隔区（rDNA-ITS）的PCR-RFLP、rDNA-ITS的PCR-SSCP和简单序列重复（SSR）标记，对12个L.基因型的遗传多样性进行了研究。ITS-SSCP揭示的多态性水平最低，其次是ITS-RFLP，然后是ISSR，SSR标记的多态性水平最高。方差分析（AMOVA）表明，大部分变异发生在种群内部。由三个共显性标记估计的近交系数值几乎相等，表明L.的生殖系统存在部分异交现象。主坐标分析（PCOA）图显示，基于采集植物的位置呈现出明显的聚类模式，这与距离隔离相吻合。研究表明，在评估L.的遗传多样性时，ITS-SSCP和ISSR标记分别比其他所用标记更具信息量。结果反映了L.的高度多样性，本研究获得的数据可用于未来的采集任务。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/3c3550444c02/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/a8aaa292558f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/26d25d4b9340/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/0f0a93bd70c5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/4ae03dc389f0/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/fa0e97af7294/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/dfac7c07fa42/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/c906a0d63669/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/3c3550444c02/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/a8aaa292558f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/26d25d4b9340/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/0f0a93bd70c5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/4ae03dc389f0/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/fa0e97af7294/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/dfac7c07fa42/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/c906a0d63669/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3956/6296603/3c3550444c02/gr8.jpg

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