Thakur V, Guptan R C, Sarin S K
Department of Gastroenterology, GB Pant Hospital, New Delhi.
J Assoc Physicians India. 2000 Aug;48(8):818-9.
Hepatitis GBV-C/HGV is a newly described RNA virus with a parenteral route of transmission. It has been implicated in fulminant hepatitis and chronic viral hepatitis. We undertook to study the prevalence of GBV-C/HGV infection in blood donors of a tertiary care hospital in India.
Serum of 221 consecutive blood donors was tested for HBsAg, anti-HCV by EIA and HGV RNA by RT-PCR. Two sets of primers; one from the 5'non-coding region and other from NS5a region of the HGV genome, were used for amplification.
Prevalence of HGV RNA was found to be very low in healthy blood donors. Only two of the 221 (0.9%) donors were found to be HGV RNA positive. HBsAg and AntiHCV were found to be present in 5.43% (12/221) and 1.31% (3/221) respectively. Dual infection was seen in two of the 221 (0.9%) patients; one patient had HBsAg and HGV RNA positivity, while the other, had HBsAg and AntiHCV positivity.
GBV-C/HGV is an uncommon infection in healthy blood donors in India, especially when compared to the prevalence of HBV and HCV infection. It is therefore unlikely to be an important cause of transfusion associated hepatitis in India.
GBV-C/HGV肝炎病毒是一种新发现的经肠道外途径传播的RNA病毒。它与暴发性肝炎和慢性病毒性肝炎有关。我们对印度一家三级护理医院的献血者中GBV-C/HGV感染的患病率进行了研究。
对连续221名献血者的血清进行乙肝表面抗原（HBsAg）、丙肝抗体（抗-HCV）的酶免疫分析（EIA）以及GBV-C/HGV RNA的逆转录聚合酶链反应（RT-PCR）检测。使用两组引物，一组来自GBV-C/HGV基因组的5'非编码区，另一组来自NS5a区，进行扩增。
健康献血者中GBV-C/HGV RNA的患病率非常低。221名献血者中只有2名（0.9%）被检测出GBV-C/HGV RNA呈阳性。乙肝表面抗原（HBsAg）和丙肝抗体（抗-HCV）的阳性率分别为5.43%（12/221）和1.31%（3/221）。221名患者中有2名（0.9%）出现双重感染；一名患者乙肝表面抗原（HBsAg）和GBV-C/HGV RNA呈阳性，另一名患者乙肝表面抗原（HBsAg）和丙肝抗体（抗-HCV）呈阳性。
在印度，GBV-C/HGV在健康献血者中是一种不常见的感染，尤其是与乙肝和丙肝感染的患病率相比。因此，它不太可能是印度输血相关肝炎的重要病因。
