Jousselin-Hosaja M, Venault P, Tobin C, Joubert C, Delacour J, Chapouthier G
Université Pierre et Marie Curie, Laboratoire de Neurobiologie des Signaux Intercellulaires, Institut des Neurosciences, UMR 7624, CNRS, 7 Quai Saint Bernard, 75252 Paris, Cedex 05, France.
Behav Brain Res. 2001 Jun;121(1-2):29-37. doi: 10.1016/s0166-4328(00)00380-6.
Immunohistochemical and behavioral techniques were used to study the effects of adrenal medulla grafts, implanted in striatum after bilateral kainic acid (KA) lesions of this structure, on the open field behavior of mice. KA-induced behavioral changes in leaning, grooming and locomotor activity of the open field test were significantly improved after grafting of the adrenal medulla, and in some respects, fully restored. Immunohistochemical identification showed that grafts contained neuron-like cells with a tyrosine hydroxylase (TH), phenylethanolamine N-methyltransferase, gamma-aminobutyric acid (GABA), choline acetyltransferase (ChAT), and enkephalin-like immunostainings. A likely interpretation of this complex pattern of results is that adrenal medullary grafts may restore the deficits of GABAergic neurons which in turn reverse the abnormalities in emotionality and locomotion. Neurobiologically, these behavioral improvements probably involve GABAergic and catecholaminergic factors of adrenal medulla grafts, although other neuroactive substances, such as acetylcholine and enkephalins, cannot be excluded.
采用免疫组织化学和行为学技术，研究在双侧纹状体经 kainic 酸（KA）损伤后将肾上腺髓质移植到纹状体中，对小鼠旷场行为的影响。在移植肾上腺髓质后，KA 诱导的旷场试验中倾斜、梳理毛发和运动活动的行为变化得到显著改善，在某些方面完全恢复。免疫组织化学鉴定显示，移植组织含有具有酪氨酸羟化酶（TH）、苯乙醇胺 N-甲基转移酶、γ-氨基丁酸（GABA）、胆碱乙酰转移酶（ChAT）和脑啡肽样免疫染色的神经元样细胞。对这一复杂结果模式的一种可能解释是，肾上腺髓质移植可能恢复了 GABA 能神经元的缺陷，进而逆转了情绪和运动方面的异常。从神经生物学角度来看，这些行为改善可能涉及肾上腺髓质移植的 GABA 能和儿茶酚胺能因素，尽管其他神经活性物质，如乙酰胆碱和脑啡肽也不能排除。
