采用高效液相色谱-蒸发光散射检测法测定西洋参中24（R）-假人参皂苷F（11）的含量。
Determination of 24(R)-pseudoginsenoside F(11) in North American ginseng using high performance liquid chromatography with evaporative light scattering detection.
作者信息
Li W, Fitzloff J F
机构信息
Functional Foods for Health (FFH) Core Analytical Laboratory, Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
出版信息
J Pharm Biomed Anal. 2001 May;25(2):257-65. doi: 10.1016/s0731-7085(00)00494-5.
A gradient liquid chromatographic method with evaporative light scattering detection (ELSD) for the determination of 24(R)-pseudoginsenoside F(11) in North American ginseng is described. Samples are analyzed by means of a reverse-phase column (Waters Spherisorb ODS-2, C(18)) using acetonitrile and water under gradient conditions as the mobile phase over 20 min. The evaporative light scattering detector (ELSD) used, was set at an evaporating temperature of 35 degrees C and nitrogen gas pressure of 3.4 bar. The detection limit (S/N>5) of 24(R)-pseudoginsenoside F(11) is 53 ng on column.
描述了一种采用蒸发光散射检测（ELSD）的梯度液相色谱法测定西洋参中24（R）-假人参皂苷F（11）的方法。样品通过反相柱（沃特世Spherisorb ODS-2，C（18））进行分析，在梯度条件下，以乙腈和水为流动相，运行20分钟。所使用的蒸发光散射检测器（ELSD）设置的蒸发温度为35℃，氮气压力为3.4巴。24（R）-假人参皂苷F（11）的柱上检测限（S/N>5）为53 ng。
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