Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam.
Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
Molecules. 2020 Jul 7;25(13):3086. doi: 10.3390/molecules25133086.
(PV), a wild species discovered in Vietnam in 1973, has been increasingly overexploited due to its economic value and therapeutic uses. This resulted in the development of PV cultivation to meet the market demand. There is little information on the accumulation of saponins in PV during cultivation, but this information could serve as an indication of the appropriate harvest time. In this study we developed an HPLC-UV/ELSD method to simultaneously determine the content of 10 characteristic saponins in PV from 2-7 years old, including G-Rb1, G-Rd, G-Rg1, G-Re, N-R1, M-R1, M-R2, V-R2, V-R11, and p-RT4. The result indicated that from 2 to 5 years, the content of saponins in PV rhizome and radix increase 3.02 and 4.2 times, respectively, whereas from 5 to 7 years, no significant changes were observed. Hence, our study suggests that after 5 years of growth could be considered as an appropriate time for PV to be harvested. Among the analyzed saponins, G-Rg1, G-Rb1, G-Rd, and especially M-R2 were the major saponins that contributed to the change of PV's saponin content through the years. In addition, the developed and validated HPLC method was proven to be reliable and effective for quality control of PV.
(PV)，1973 年在越南发现的一种野生物种，由于其经济价值和治疗用途而被过度开发。这导致了 PV 种植的发展以满足市场需求。关于 PV 在种植过程中皂素积累的信息很少，但这些信息可以作为适当收获时间的指示。在这项研究中，我们开发了一种 HPLC-UV/ELSD 方法，可同时测定 2-7 年生 PV 中 10 种特征皂素的含量，包括 G-Rb1、G-Rd、G-Rg1、G-Re、N-R1、M-R1、M-R2、V-R2、V-R11 和 p-RT4。结果表明，从 2 年到 5 年，PV 根茎和根中的皂素含量分别增加了 3.02 倍和 4.2 倍，而从 5 年到 7 年，没有观察到明显的变化。因此，我们的研究表明，生长 5 年后可以考虑作为 PV 收获的合适时间。在所分析的皂素中，G-Rg1、G-Rb1、G-Rd，尤其是 M-R2 是导致 PV 皂素含量多年来变化的主要皂素。此外，所开发和验证的 HPLC 方法被证明是可靠和有效的，可用于 PV 的质量控制。
