Griparic L, van der Bliek A M
Department of Biological Chemistry, University of California, Los Angeles CA 90095, USA.
Traffic. 2001 Apr;2(4):235-44. doi: 10.1034/j.1600-0854.2001.1r008.x.
The roles of mitochondria in cell death and in aging have generated much excitement in recent years. At the same time, however, a quiet revolution in our thinking about mitochondrial ultrastructure has begun. This revolution started with the use of vital dyes and of green fluorescent protein fusion proteins, showing that mitochondria are very dynamic structures that constantly move, divide and fuse throughout the life of a cell. More recently, some of the first proteins contributing to these various processes have been discovered. Our view of the internal structures of mitochondria has also changed. Three-dimensional reconstructions obtained with high voltage electron microscopy show that cristae are often connected to the mitochondrial inner membrane by thin tubules. These new insights are brought to bear on the wealth of data collected by conventional electron microscopic analysis.
近年来，线粒体在细胞死亡和衰老过程中的作用引发了广泛关注。然而与此同时，我们对线粒体超微结构的认识也在悄然发生变革。这场变革始于活体染料和绿色荧光蛋白融合蛋白的应用，结果表明线粒体是极具动态性的结构，在细胞整个生命周期中持续进行移动、分裂和融合。最近，一些参与这些不同过程的首批蛋白质已被发现。我们对线粒体内在结构的看法也有所改变。通过高压电子显微镜获得的三维重建结果显示，嵴通常通过细管与线粒体内膜相连。这些新见解被应用于常规电子显微镜分析所收集的大量数据。
