通过二维聚丙烯酰胺凝胶电泳分离大鼠肝脏内质网膜蛋白。
The separation of rat liver endoplasmic reticulum membrane proteins by two dimensional polyacrylamide gel electrophoresis.
作者信息
Czosnek H H, Hochberg A A
出版信息
Mol Biol Rep. 1975 Mar;2(1):19-25. doi: 10.1007/BF00357293.
PMID:1128514
Abstract
The separation of rat liver endoplasmic reticulum membrane proteins by two dimensional polyacrylamide gel electrophoresis is described. By this method, the proteins of the rough membrane ribosomes could be separated from the other rough membrane proteins. Both rough and smooth membrane fractions contain at least 30 defined membranal proteins. The electro-phoretic patterns of rough and smooth membrane proteins are clearly different.
摘要
本文描述了通过二维聚丙烯酰胺凝胶电泳分离大鼠肝脏内质网膜蛋白的方法。通过这种方法，可以将糙面核糖体膜蛋白与其他糙面膜蛋白分离开来。糙面和滑面膜部分均至少含有30种明确的膜蛋白。糙面和滑面膜蛋白的电泳图谱明显不同。
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