对天然大鼠肝脏粗面内质网及其体外重构物的氨基酸掺入活性的研究。
Studies on the amino acid-incorporating activity of native rat liver rough membrane and that reconstituted in vitro.
作者信息
De Groot N, Yuli I J, Czosnek H, Shiklosh Y, Hochberg A A
出版信息
Biochem J. 1976 Jul 15;158(1):23-31. doi: 10.1042/bj1580023.
Abstract
The amino acid-incorporating activities of free polyribosomes, rough membranes and rough membranes reconstituted in vitro, derived from rat liver, were compared. The amino acid-incorporating activity of the two membrane fractions were very similar in their response towards changes in pH, Mg2+ concentration and temperature, but differed from the response of the amino acid-incorporating activity of free polyribosomes. Free polyribosomes irreversibly lost part of their amino acid-incorporating capacity after they had become bound to rough membrane, from which the original ribosomes had been removed. Ribonuclease activity present in the membrane fraction may be responsible for this loss.
摘要
比较了来源于大鼠肝脏的游离多核糖体、糙面内质网以及体外重建的糙面内质网的氨基酸掺入活性。两种膜组分的氨基酸掺入活性在对pH、Mg2+浓度和温度变化的反应方面非常相似，但与游离多核糖体的氨基酸掺入活性的反应不同。游离多核糖体在与已去除原始核糖体的糙面内质网结合后，不可逆地丧失了部分氨基酸掺入能力。膜组分中存在的核糖核酸酶活性可能是造成这种损失的原因。
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