Cárdenas E, Munstermann L E, Martínez O, Corredor D, Ferro C
Laboratorio de Entomología, Instituto Nacional de Salud, Bogotá, Colombia.
Mem Inst Oswaldo Cruz. 2001 Feb;96(2):189-96. doi: 10.1590/s0074-02762001000200010.
Polyacrylamide gel electrophoresis was used to elucidate genetic variation at 13 isozyme loci among forest populations of Lutzomyia shannoni from three widely separated locations in Colombia: Palambí (Nariño Department), Cimitarra (Santander Department) and Chinácota (Norte de Santander Department). These samples were compared with a laboratory colony originating from the Magdalena Valley in Central Colombia. The mean heterozygosity ranged from 16 to 22%, with 2.1 to 2.6 alleles detected per locus. Nei's genetic distances among populations were low, ranging from 0.011 to 0.049. The estimated number of migrants (Nm=3.8) based on Wright's F-Statistic, F ST, indicated low levels of gene flow among Lu. shannoni forest populations. This low level of migration indicates that the spread of stomatitis virus occurs via infected host, not by infected insect. In the colony sample of 79 individuals, the Gpi locus was homozygotic (0.62/0.62) in all females and heterozygotic (0.62/0.72) in all males. Although this phenomenon is probably a consequence of colonization, it indicates that Gpi is linked to a sex determining locus.
采用聚丙烯酰胺凝胶电泳法，对来自哥伦比亚三个相距甚远地点（帕兰比（纳里尼奥省）、西米塔拉（桑坦德省）和奇纳科塔（北桑坦德省））的香农白蛉森林种群的13个同工酶位点的遗传变异进行了研究。将这些样本与源自哥伦比亚中部马格达莱纳河谷的实验室群体进行了比较。平均杂合度在16%至22%之间，每个位点检测到2.1至2.6个等位基因。种群间的内氏遗传距离较低，范围为0.011至0.049。根据赖特F统计量FST估计的迁移个体数（Nm = 3.8）表明，香农白蛉森林种群间的基因流动水平较低。这种低水平的迁移表明，口炎病毒的传播是通过受感染的宿主，而非受感染的昆虫。在79个个体的群体样本中，Gpi位点在所有雌性个体中均为纯合子（0.62/0.62），在所有雄性个体中均为杂合子（0.62/0.72）。尽管这种现象可能是定殖的结果，但它表明Gpi与一个性别决定位点相连。
