Mukhopadhyay J, Ghosh K, Ferro C, Munstermann L E
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520-8034, USA.
J Med Entomol. 2001 Mar;38(2):260-7. doi: 10.1603/0022-2585-38.2.260.
Genetic variability of eight Colombian field populations and two laboratory colonies of a tropical forest sand fly, Lutzomyia shannoni Dyar, was assessed by comparing allozyme frequencies at 20 enzyme loci. Substantial genetic variability was noted in all strains, with mean heterozygosities of 13-21% and alleles per locus of 2.0-2.8. Four loci were monomorphic. Six populations in north and central Colombia showed close genetic similarity (Nei's distances, 0.01-0.09), despite mountainous environment, discontinuous forest habitat, and elevation differences from 125 to 1,220 m. Two samples representing the Orinoco (near Villavicencio) and Amazon (near Leticia) river basins were similar (Nei's distance, 0.08) but diverged substantially from the central six samples (Nei's distances, 0.26-0.40). Although the range of L. shannoni extends from the southeastern United States to northern Argentina, three genetically distinct, geographically discrete, groups were discerned by the current analysis: Orinoco-Amazon river basins, north-central Colombia, and eastern United States.
通过比较20个酶位点的等位酶频率，评估了热带森林沙蝇——香农白蛉（Lutzomyia shannoni Dyar）的8个哥伦比亚野外种群和2个实验室群体的遗传变异性。在所有菌株中均观察到显著的遗传变异性，平均杂合度为13%-21%，每个位点的等位基因数为2.0-2.8。有4个位点是单态的。尽管存在山区环境、不连续的森林栖息地以及海拔从125米到1220米的差异，但哥伦比亚北部和中部的6个种群显示出密切的遗传相似性（内氏距离，0.01-0.09）。代表奥里诺科河（靠近比亚维森西奥）和亚马逊河（靠近莱蒂西亚）流域的两个样本相似（内氏距离，0.08），但与中部的6个样本有很大差异（内氏距离，0.26-0.40）。尽管香农白蛉的分布范围从美国东南部延伸到阿根廷北部，但通过当前分析可识别出三个遗传上不同、地理上离散的群体：奥里诺科-亚马逊河流域、哥伦比亚中北部和美国东部。
