Kamal Mustafa, Yasmeen Ghazala, Saher Noor Us, Naz Farah, Rafiq Naseem, Khan Wali, Iqbal Muhammad Anwar, Kabir Muhammad, Mushtaq Safia, Hassan Habib Ul, Usman Tahir
Department of Zoology, Abdul Wali Khan University, Mardan 23200, Pakistan.
Department of Zoology, University of Karachi, Karachi 75270, Pakistan.
Saudi J Biol Sci. 2022 Mar;29(3):1694-1698. doi: 10.1016/j.sjbs.2021.10.062. Epub 2021 Oct 29.
The study was planned to evaluate the inter, and intra population genetic variation in general protein banding pattern in larvae, by using 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The larvae were collected from slaughtered goats head from five different locations (AAS, PN, LA, GM, and BC) of Karachi, Pakistan. The data obtained was subjected to POPGENE (Population Genetic Analysis) software for analysis. The polymorphic loci within populations ranged from 45.45% to 90.91%. Polymorphic loci observed in all populations were 90.91%. The expected heterozygosity observed was 0.182 ± 0.096 in all populations. The chi-square test showed 5 out of 11 loci at H-W equilibrium. The overall fixation index (FST) value was 0.108, showing that the likelihood of subpopulations being differentiated from one another is about 11 percent. The gene flow value (Nm = 2.065) was higher, showing that genes flow occurs between populations. The values of genetic identity were greater, and genetic distance were smaller among all the populations, which means that all the populations were more alike and closer to each other. It was concluded that there was no sympatric and parapatric population differentiation observed among all the population of and the populations of the five different locations were not genetically and reproductively isolated from each other.
本研究旨在通过使用10%十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）来评估幼虫群体间和群体内一般蛋白质条带模式的遗传变异。幼虫采自巴基斯坦卡拉奇五个不同地点（AAS、PN、LA、GM和BC）屠宰山羊的头部。将获得的数据用POPGENE（群体遗传分析）软件进行分析。群体内的多态位点范围为45.45%至90.91%。在所有群体中观察到的多态位点为90.91%。在所有群体中观察到的预期杂合度为0.182±0.096。卡方检验显示，11个位点中有5个处于哈迪-温伯格平衡。总体固定指数（FST）值为0.108，表明亚群体彼此分化的可能性约为11%。基因流值（Nm = 2.065）较高，表明群体间存在基因流动。所有群体之间的遗传同一性值较大，遗传距离较小，这意味着所有群体彼此更相似且关系更密切。得出的结论是，在所有群体中未观察到同域和邻域群体分化，五个不同地点的群体在遗传和生殖上并未彼此隔离。
