Chang J L, Tsao Y P, Liu D W, Huang S J, Lee W H, Chen S L
Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, ROC.
J Biomed Sci. 2001 Mar-Apr;8(2):206-13. doi: 10.1007/BF02256414.
To investigate the expression of human papillomavirus type 16 (HPV-16) E5 protein in squamous neoplastic changes in the uterine cervix, the specific E5 antibody was generated and used to identify the expression of E5 protein in 40 cases of HPV-16-positive tissues and 5 previously identified HPV-negative normal cervical tissues. The results revealed that E5 protein was primarily expressed in the lower third of the epithelium in low-grade squamous intraepithelial lesions (SILs) and throughout the whole epithelium in high-grade SILs. In invasive squamous carcinoma, 60% of HPV-16-infected cancers which contained the episomal viral genome had the E5 gene, and could express E5 protein which was located throughout the whole epithelium. Previously, we documented the expression of type I growth factor receptors [ERBB1/EGFR (epidermal growth factor receptor), ERBB2, ERBB3 and ERBB4] in the full range of cervical neoplasias by immunohistochemistry assay. Hence, in this study, we extensively analyzed the correlation between the expression of E5 protein and the expression of type I growth factor receptors. Among 40 HPV-16- infected cervical neoplasias, we found that the expression of E5 protein was significantly correlated with either the expression of the ERBB1 or the ERBB4 receptor.
为研究16型人乳头瘤病毒（HPV-16）E5蛋白在子宫颈鳞状上皮肿瘤性病变中的表达情况，制备了特异性E5抗体，并用于鉴定40例HPV-16阳性组织及5例先前鉴定为HPV阴性的正常子宫颈组织中E5蛋白的表达。结果显示，E5蛋白主要在低级别鳞状上皮内病变（SIL）上皮的下三分之一处表达，而在高级别SIL中则在上皮全层表达。在浸润性鳞状细胞癌中，60%含有游离病毒基因组的HPV-16感染癌具有E5基因，并能表达位于上皮全层的E5蛋白。此前，我们通过免疫组织化学检测记录了I型生长因子受体[ERBB1/表皮生长因子受体（EGFR）、ERBB2、ERBB3和ERBB4]在整个子宫颈肿瘤中的表达情况。因此，在本研究中，我们广泛分析了E5蛋白表达与I型生长因子受体表达之间存在的相关性。在40例HPV-16感染的子宫颈肿瘤中，我们发现E5蛋白的表达与ERBB1或ERBB4受体的表达显著相关。
