基因扩增与毛细管凝胶电泳在聚二甲基硅氧烷-玻璃混合微芯片上的集成。

Integration of gene amplification and capillary gel electrophoresis on a polydimethylsiloxane-glass hybrid microchip.

作者信息

Hong J W, Fujii T, Seki M, Yamamoto T, Endo I

机构信息

Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Japan.

出版信息

Electrophoresis. 2001 Jan;22(2):328-33. doi: 10.1002/1522-2683(200101)22:2<328::AID-ELPS328>3.0.CO;2-C.

DOI:10.1002/1522-2683(200101)22:2<328::AID-ELPS328>3.0.CO;2-C

PMID:11288901

Abstract

We report on the development of a hybrid polydimethylsiloxane (PDMS)-glass microchip for genetic analysis by functional integration of polymerase chain reaction (PCR) and capillary gel electrophoresis (CGE), and on related temperature control systems for PCR on a PDMS-glass hybrid microchip. The microchip was produced by molding PDMS against a microfabricated master with comparatively simple and inexpensive methods. PCR was successfully carried out on the PDMS-glass hybrid microchip with 500 bp target of lambdaDNA and the amplified gene was subsequently analyzed by CGE on the same PDMS-glass microchip. The chip could be considered as an inexpensive single-use apparatus compared to glass or silicon-made microchips for the same purpose.

摘要

我们报告了一种用于基因分析的混合聚二甲基硅氧烷（PDMS）-玻璃微芯片的开发，该芯片通过聚合酶链反应（PCR）和毛细管凝胶电泳（CGE）的功能集成实现，还报告了用于在PDMS-玻璃混合微芯片上进行PCR的相关温度控制系统。该微芯片是通过相对简单且廉价的方法，使用微加工母模对PDMS进行模塑制成的。在PDMS-玻璃混合微芯片上成功对λDNA的500 bp靶标进行了PCR，随后在同一PDMS-玻璃微芯片上通过CGE对扩增的基因进行了分析。与用于相同目的的玻璃或硅制微芯片相比，该芯片可被视为一种廉价的一次性装置。