对从菲律宾登革热患者采集的血清样本进行IgM捕获酶联免疫吸附测定。
IgM-capture ELISA of serum samples collected from Filipino dengue patients.
作者信息
Buerano C C, Ibrahim I N, Contreras R C, Hasebe F, Matias R R, Natividad F F, Igarashi A
机构信息
Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City.
出版信息
Southeast Asian J Trop Med Public Health. 2000 Sep;31(3):524-9.
Viral antigens for 4 dengue serotypes were produced in C6/36 Aedes albopictus cells. These were used as assay antigens for IgM-capture ELISA to detect IgM antibodies in sera of dengue patients from 3 hospitals in Metro Manila, Philippines. A total of 378 serum samples came from National Children's Hospital (NCH), San Lazaro Hospital (SLH), and St Luke's Medical Center (SLMC), from January to November 1995. Three hundred and four (304) out of 378 serum samples, or 80.42% showed positive IgM ELISA titer against at least one of the 4 assay antigens. Dengue type 4 (D4) antigen detected antibodies in 61.90% (234/378) of these serum samples, whereas type 1 (D1), type 3 (D3), and type 2 (D2) had detection rates of 60.05% (227/378), 50.79% (192/378) and 49.47% (187/378) respectively. Although the results show that both D1 and D4 are the most effective antigens in identifying dengue infections for this batch of samples, the use of a cocktail of antigens is still recommended. The results of this study are the basis for the IgM-capture ELISA protocol presently applied for the laboratory confirmation of dengue cases in the Philippines.
4种登革热血清型的病毒抗原在白纹伊蚊C6/36细胞中产生。这些抗原被用作IgM捕获ELISA的检测抗原，以检测来自菲律宾马尼拉大都会3家医院的登革热患者血清中的IgM抗体。1995年1月至11月，共有378份血清样本分别来自国立儿童医院（NCH）、圣拉萨罗医院（SLH）和圣卢克医疗中心（SLMC）。378份血清样本中有304份（80.42%）针对至少一种检测抗原的IgM ELISA滴度呈阳性。在这些血清样本中，登革热4型（D4）抗原检测到抗体的比例为61.90%（234/378），而1型（D1）、3型（D3）和2型（D2）的检测率分别为60.05%（227/378）、50.79%（192/378）和49.47%（187/378）。虽然结果表明D1和D4都是这批样本中鉴定登革热感染最有效的抗原，但仍建议使用抗原混合物。本研究结果是目前菲律宾用于登革热病例实验室确诊的IgM捕获ELISA方案的依据。
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