Cardosa M J, Tio P H, Nimmannitya S, Nisalak A, Innis B
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang.
Southeast Asian J Trop Med Public Health. 1992 Dec;23(4):726-9.
The highly sensitive AFRIMS format IgM capture ELISA for the diagnosis of dengue virus infections requires the use of mouse brain derived hemagglutinins and consequently also the use of 20% acetone extracted normal human serum to eliminate high background. These reagents are not always easily available and we have thus compared the AFRIMS format with another published format which uses cell culture derived antigens (culture fluid, CF, format) in order to determine if it is reasonable to use cell culture derived antigens in situations where hemagglutinins and normal human serum are difficult to obtain. The study shows that using AFRIMS results as the reference point, the CF format described here has a sensitivity of 90% and a specificity of 96%.
用于诊断登革病毒感染的高灵敏度AFRIMS格式IgM捕获ELISA需要使用源自鼠脑的血凝素，因此还需要使用20%丙酮提取的正常人血清来消除高背景。这些试剂并非总是容易获得，因此我们将AFRIMS格式与另一种已发表的使用细胞培养衍生抗原（培养液，CF，格式）的格式进行了比较，以确定在难以获得血凝素和正常人血清的情况下使用细胞培养衍生抗原是否合理。研究表明，以AFRIMS结果作为参考点，此处描述的CF格式灵敏度为90%，特异性为96%。
