Nagai H, Murakami Y, Morita Y, Yokoyama K, Tamiya E
School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa.
Anal Chem. 2001 Mar 1;73(5):1043-7. doi: 10.1021/ac000648u.
A microchamber array for PCR was developed by semiconductor microfabrication technology. The microchambers were designed to be of picoliter quantity. To optimize fluid retention, the surface states of the substrate and the inner walls were examine for four different types of microchamber. The substrate was silicon, while silicon dioxide was selected for the inner walls. PCR was performed in the microchamber array, and the amplification of DNA was detected using a technique based on the energy transfer of fluorescent dyes. The lower volume limit for PCR was investigated using various sizes of microchambers. Microchambers with volume greater than 86 pL gave successful PCR. In addition, the system was improved in order to take up the PCR product. To prevent mixing of the samples, the samples were dried after PCR using a membrane that permeates only vapor.
通过半导体微加工技术开发了一种用于聚合酶链反应（PCR）的微腔阵列。这些微腔被设计为皮升量级。为了优化液体保留，针对四种不同类型的微腔研究了基底和内壁的表面状态。基底为硅，而内壁选用二氧化硅。在微腔阵列中进行PCR，并使用基于荧光染料能量转移的技术检测DNA的扩增。使用各种尺寸的微腔研究了PCR的下限体积。体积大于86皮升的微腔实现了成功的PCR。此外，对该系统进行了改进以收集PCR产物。为防止样品混合，PCR后使用仅能透过蒸汽的膜将样品干燥。
