Johansen C A, van den Hurk A F, Ritchie S A, Zborowski P, Nisbet D J, Paru R, Bockarie M J, Macdonald J, Drew A C, Khromykh T I, Mackenzie J S
Department of Microbiology and Parasitology, The University of Queensland, Brisbane, Australia.
Am J Trop Med Hyg. 2000 May;62(5):631-8. doi: 10.4269/ajtmh.2000.62.631.
After Japanese encephalitis (JE) virus emerged in the Torres Strait in Australia in 1995, investigations were initiated into the origin of the incursion. New Guinea was considered the most likely source, given its proximity to islands of the Torres Strait. Almost 400,000 adult mosquitoes were processed for virus isolation from 26 locations in the Western Province of Papua New Guinea (PNG) between February 1996 and February 1998, yielding three isolates of JE virus. Two isolates of Murray Valley encephalitis, 17 isolates of Sindbis, and 1 each of Sepik and Ross River viruses were also obtained. Nucleic acid sequences of the PNG JE isolates were determined in the prM region, and in a region overlapping a part of the fifth nonstructural protein and the 3' untranslated region. The PNG isolates belonged to genotype II, and shared > 99.2% identity with isolates from humans and mosquitoes from the Torres Strait, suggesting that PNG is the source of incursions of JE virus into Australia.
1995年日本脑炎（JE）病毒在澳大利亚的托雷斯海峡出现后，人们开始对此次病毒入侵的起源展开调查。考虑到新几内亚岛与托雷斯海峡诸岛距离较近，它被认为是最有可能的源头。1996年2月至1998年2月期间，从巴布亚新几内亚（PNG）西部省份的26个地点采集了近40万只成年蚊子用于病毒分离，从中分离出三株JE病毒。还获得了两株墨累谷脑炎病毒、17株辛德毕斯病毒以及各1株塞皮克病毒和罗斯河病毒。测定了PNG地区JE病毒分离株在prM区域以及与第五个非结构蛋白部分和3'非翻译区重叠区域的核酸序列。PNG地区的分离株属于基因型II，与来自托雷斯海峡人类和蚊子的分离株具有> 99.2%的同一性，这表明PNG是JE病毒入侵澳大利亚的源头。
