在澳大利亚从环喙库蚊中分离出日本脑炎病毒。

Isolation of Japanese encephalitis virus from Culex annulirostris in Australia.

作者信息

Ritchie S A, Phillips D, Broom A, Mackenzie J, Poidinger M, van den Hurk A

机构信息

Tropical Public Health Unit, Cairns, Queensland, Australia.

出版信息

Am J Trop Med Hyg. 1997 Jan;56(1):80-4. doi: 10.4269/ajtmh.1997.56.80.

DOI:10.4269/ajtmh.1997.56.80

PMID:9063367

Abstract

Mosquitoes were collected using CO2 and octenol-baited light traps during an outbreak of Japanese encephalitis (JE) on Badu Island in the Torres Strait, Australia in April 1995. A total of 13,300 mosquitoes comprising 12 species were processed for virus isolation. Eight isolates of JE virus were obtained from Culex annulirostris, with a carriage rate of 2.97:1,000; this mosquito also yielded one Sindbis virus isolate. A reverse transcriptase-polymerase chain reaction was used to sequence the JE viruses, which were compared with published sequences. The eight isolates were 90% homologous with known JE strains but only 68% homologous with other flaviviruses. Among the isolates, 99% homology was obtained, suggesting a common point of origin.

摘要

1995年4月在澳大利亚托雷斯海峡巴杜岛发生日本脑炎（JE）疫情期间，使用二氧化碳和诱蚊烯诱饵诱蚊灯捕蚊器收集蚊子。总共处理了13300只蚊子，分属12个物种以进行病毒分离。从环喙库蚊中获得了8株日本脑炎病毒分离株，携带率为2.97:1000；这种蚊子还产生了1株辛德毕斯病毒分离株。使用逆转录聚合酶链反应对日本脑炎病毒进行测序，并与已发表的序列进行比较。这8株分离株与已知的日本脑炎毒株有90%的同源性，但与其他黄病毒只有68%的同源性。在这些分离株中，同源性达到99%，表明有共同的起源点。