Goldfarb A N, Wong D, Racke F K
Department of Pathology, University of Virginia Health Sciences Center, HSC Box 204, Charlottesville, VA 22908, USA.
Am J Pathol. 2001 Apr;158(4):1191-8. doi: 10.1016/S0002-9440(10)64068-0.
In myelodysplasias and acute myeloid leukemias, abnormalities in erythroid development often parallel abnormalities in megakaryocytic development. Erythroleukemic cells in particular have been shown to possess the potential to undergo megakaryocytic differentiation in response to a variety of stimuli. Whether or not such lineage plasticity occurs as a consequence of the leukemic phenotype has not previously been addressed. In this study, highly purified primary human erythroid progenitors were subjected to stimuli known to induce megakaryocytic differentiation in erythroleukemic cells. Remarkably, the primary erythroid progenitors rapidly responded with morphological and immunophenotypic evidence of megakaryocytic differentiation, equivalent to that seen in erythroleukemic cells. Even erythroblasts expressing high levels of hemoglobin manifested partial megakaryocytic differentiation. These results indicate that the lineage plasticity observed in erythroleukemic cells reflects an intrinsic property of cells in the erythroid lineage rather than an epiphenomenon of leukemic transformation.
在骨髓增生异常综合征和急性髓系白血病中，红系发育异常往往与巨核系发育异常并存。特别是红白血病细胞已被证明有潜力在多种刺激下发生巨核系分化。此前尚未探讨过这种谱系可塑性是否是白血病表型的结果。在本研究中，对高度纯化的原代人红系祖细胞施加已知可诱导红白血病细胞发生巨核系分化的刺激。值得注意的是，原代红系祖细胞迅速出现巨核系分化的形态学和免疫表型证据，与红白血病细胞中的情况相当。即使是表达高水平血红蛋白的成红细胞也表现出部分巨核系分化。这些结果表明，在红白血病细胞中观察到的谱系可塑性反映了红系谱系细胞的内在特性，而非白血病转化的附带现象。
