Nunes D N, Kowalski L P, Simpson A J
Laboratory of Cancer Genetics, Ludwig Institute for Cancer Research, 010509-011 São Paulo, SP Brazil.
Int J Cancer. 2001 Apr 15;92(2):214-9. doi: 10.1002/1097-0215(200102)9999:9999<::aid-ijc1176>3.0.co;2-c.
A series of eight microsatellite loci were assayed for both loss of heterozygosity and new mutated alleles in 91 head and neck squamous cell carcinomas. In 58 cases, alterations were detected and used as markers for assaying the presence of circulating tumor-derived DNA in the patients' plasma. This was unambiguously detected in 17 cases. The probability of detecting circulating DNA was independent of tumor stage and was found to be present even in some individuals with stage I tumors. The presence of such DNA, however, could not be correlated with disease outcome or other significant clinical parameters, suggesting that it has no prognostic significance. The results indicate that circulating tumor-derived DNA could be used as a means of early diagnosis of head and neck tumors.
对91例头颈部鳞状细胞癌检测了一系列8个微卫星位点的杂合性缺失和新的突变等位基因。在58例病例中检测到改变，并将其用作检测患者血浆中循环肿瘤衍生DNA存在的标志物。在17例病例中明确检测到了这种DNA。检测循环DNA的概率与肿瘤分期无关，甚至在一些I期肿瘤患者中也发现存在循环DNA。然而，这种DNA的存在与疾病预后或其他重要临床参数无关，表明它没有预后意义。结果表明，循环肿瘤衍生DNA可作为头颈部肿瘤早期诊断的一种手段。
