大鼠肾脏己糖激酶的组织化学测定
Histochemical measurements of rat kidney hexokinase.
作者信息
Brannan T S, Corder C N, Rizk M
出版信息
Proc Soc Exp Biol Med. 1975 Mar;148(3):714-9. doi: 10.3181/00379727-148-38616.
PMID:1129291
Abstract
HK has been measured in substructures of the developing rat nephron. Reactions were carried on samples dissected from freeze dried kidney and assayed in 1 mul reaction volume utilizing the "oil-well" technique. Samples from 4 days prenatal to 44-day adult were analyzed. Activity during development decreased in G, PCT, and it increased in ALH. There was little change in PRT, DCT and CT. Activity in small arteries decreased.
摘要
已对发育中的大鼠肾单位的亚结构进行了己糖激酶（HK）的测定。反应在从冷冻干燥的肾脏中解剖出的样本上进行，并使用“油井”技术在1微升反应体积中进行测定。分析了从产前4天到成年44天的样本。发育过程中，肾小球（G）、近端小管（PCT）中的活性降低，而醛固酮分泌细胞（ALH）中的活性增加。远曲小管（PRT）、致密斑（DCT）和连接小管（CT）中的活性变化不大。小动脉中的活性降低。
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