Yao Y, Ho P, Yeung W S
Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital, Hong Kong, China.
Fertil Steril. 2000 Apr;73(4):680-6. doi: 10.1016/s0015-0282(99)00637-8.
To investigate the capacitation and motility kinetics of spermatozoa treated with human follicular fluid (FF).
Controlled, experimental laboratory study.
University-based gynecology unit.
PATIENT(S): Human FF was collected from women undergoing assisted reproductive treatment. Semen samples were obtained from men visiting subfertility clinics.
INTERVENTION(S): Spermatozoa were incubated with human FF under various experimental conditions. Spermatozoa incubated with Earle's balanced salt solution were used as the control.
MAIN OUTCOME MEASURE(S): Chlortetracycline staining patterns and sperm motility parameters.
RESULT(S): The rate of capacitation in the human FF-treated spermatozoa was significantly higher than that in the control spermatozoa after 1 hour and 3 hours of treatment. The percentage of acrosome-reacted spermatozoa also was significantly higher after human FF treatment than after control treatment. These effects of human FF were dose-dependent. Human FF-treated spermatozoa maintained their velocities at the zero-hour level for 5 hours, whereas the velocities of the control spermatozoa decreased significantly after 1 hour. Human FF treatment significantly increased the beat cross-frequency above the rate at zero hour for 5 hours. The hyperactivation of the human FF-treated spermatozoa remained stable for 3 hours, whereas that of the control spermatozoa decreased significantly after 1 hour of incubation. Significantly more human FF-treated spermatozoa underwent hyperactivation than did control spermatozoa after 1 hour and 3 hours of treatment. The effects of human FF on beat cross-frequency and hyperactivation were dose-dependent.
CONCLUSION(S): Human FF promotes capacitation and the acrosome reaction within a short period. It also stimulates or maintains various sperm motility parameters.
研究经人卵泡液（FF）处理的精子的获能及运动动力学。
对照实验性实验室研究。
大学附属妇科单位。
人卵泡液取自接受辅助生殖治疗的女性。精液样本取自就诊于不育门诊的男性。
精子在各种实验条件下与人卵泡液孵育。用Earle平衡盐溶液孵育的精子用作对照。
金霉素染色模式和精子运动参数。
处理1小时和3小时后，经人卵泡液处理的精子的获能率显著高于对照精子。人卵泡液处理后顶体反应精子的百分比也显著高于对照处理后。人卵泡液的这些作用呈剂量依赖性。经人卵泡液处理的精子在5小时内保持其零小时水平的速度，而对照精子的速度在1小时后显著下降。人卵泡液处理使拍击交叉频率在零小时速率之上显著增加并持续5小时。经人卵泡液处理的精子的超活化在3小时内保持稳定，而对照精子在孵育1小时后超活化显著下降。处理1小时和3小时后，经人卵泡液处理的精子发生超活化的数量显著多于对照精子。人卵泡液对拍击交叉频率和超活化的作用呈剂量依赖性。
人卵泡液在短时间内促进精子获能和顶体反应。它还刺激或维持各种精子运动参数。
