生长抑素的放射免疫测定法。

Radioimmunoassay for GH-release inhibiting hormone.

作者信息

Arimura A, Sato H, Coy D H, Schally A V

出版信息

Proc Soc Exp Biol Med. 1975 Mar;148(3):784-9. doi: 10.3181/00379727-148-38631.

Abstract

The synthetic growth hormone release inhibiting hormone (GH-RIH) was conjugated with human serum globulin using glutaraldehyde and administered to rabbits. An antiserum thus generated 70% of 125I-Tyr1-GH-RIH. The binding was inhibited by unlabelled GH-RIH and the inhibition was dose-related, enabling us to establish a radioimmunoassay method for GH-RIH. The minimum detectable dose was 4 pg. A linearity was demonstrated for immunoreactive GH-RIH of extracts of rat and pig hypothalami, indicating that they contained substance(s) indistinguishable from GH-RIH. Various hypothalamic and pituitary hormones did not interfere with the radioimmunoassay, but considerable cross-reaction was observed for linear GH-RIH and the ring portion of GIH-RIH, suggesting that the antigenic determinant involved the amino acid sequence from position 3-14 of GH-RIH or part of it. Plasma protein appears to contain substance(s) immunologically indistinguishable from GH-RIH or to interfere, in a nonspecific manner, with the radioimmunoassay system for GH-RIH.

摘要

使用戊二醛将合成的生长激素释放抑制激素（GH - RIH）与人血清球蛋白偶联，并将其注射给兔子。由此产生的抗血清能结合70%的125I - Tyr1 - GH - RIH。未标记的GH - RIH可抑制这种结合，且抑制作用呈剂量相关，这使我们能够建立一种用于GH - RIH的放射免疫分析方法。最低检测剂量为4皮克。大鼠和猪下丘脑提取物中免疫反应性GH - RIH呈现线性关系，表明它们含有与GH - RIH难以区分的物质。各种下丘脑和垂体激素不干扰放射免疫分析，但观察到线性GH - RIH和GIH - RIH的环部分存在相当程度的交叉反应，这表明抗原决定簇涉及GH - RIH第3至14位的氨基酸序列或其一部分。血浆蛋白似乎含有与GH - RIH在免疫上难以区分的物质，或者以非特异性方式干扰用于GH - RIH的放射免疫分析系统。