Ito Y, Ikai Y, Oka H, Matsumoto H, Miyazaki Y, Takeba K, Nagase H
Aichi Prefectural Institute of Public Health, Nagoya, Japan.
J Chromatogr A. 2001 Mar 16;911(2):217-23. doi: 10.1016/s0021-9673(01)00512-x.
A multiresidue analytical method was developed for the confirmation of benzylpenicillin (PCG), phenoxymethylpenicillin (PCV), oxacillin (MPIPC), cloxacillin (MCIPC), nafcillin (NFPC) and dicloxacillin (MDIPC) in bovine tissues using electrospray ionization liquid chromatography-tandem mass spectrometry (LC-ESI-MS-MS) with a product ion scan mode. All penicillins gave [M-H]-, [M-H-CO2]- and [M-H-141]- as the product ion, when [M-H]- was selected as the precursor ion. Combination of an ion-exchange cartridge clean-up and the LC-ESI-MS-MS method can reliably identify all of these penicillins fortified at a concentration of 0.05 mg/kg in bovine tissues, including liver, kidney and muscle. The limits of confirmation satisfy the maximum residue limits for each of the penicillins established by the World Health Organization, US Food and Drug Administration, European Union and Japan.
采用电喷雾电离液相色谱 - 串联质谱法（LC - ESI - MS - MS）的产物离子扫描模式，建立了一种多残留分析方法，用于确证牛组织中苄青霉素（PCG）、苯氧甲基青霉素（PCV）、苯唑西林（MPIPC）、氯唑西林（MCIPC）、萘夫西林（NFPC）和双氯西林（MDIPC）。当选择[M - H]-作为母离子时，所有青霉素的产物离子均为[M - H]-、[M - H - CO₂]-和[M - H - 141]-。离子交换柱净化与LC - ESI - MS - MS方法相结合，能够可靠地鉴定牛组织（包括肝脏、肾脏和肌肉）中添加浓度为0.05 mg/kg的所有这些青霉素。确证限符合世界卫生组织、美国食品药品监督管理局、欧盟和日本制定的各青霉素最大残留限量标准。
