Blanchflower W J, Hewitt A S, Kennedy D G
Department of Agriculture, Veterinary Sciences Division, Belfast, UK.
Analyst. 1994 Dec;119(12):2595-601. doi: 10.1039/an9941902595.
A confirmatory assay using liquid chromatography-electrospray mass spectrometry (LC-EMS) is presented for the simultaneous determination of penicillins V and G along with oxacillin, cloxacillin and dicloxacillin in muscle, kidney and milk. Nafcillin is used as an internal standard. Samples are extracted using acetonitrile and cleaned up using liquid-liquid extraction into dichloromethane. The extracts are injected into a dedicated LC-EMS instrument and any penicillins present are detected using multiple single-ion monitoring. Three or four ions can be detected for each penicillin, meeting one of the recommended EU criteria for confirmatory residue analyses using low-resolution mass spectrometry. The detection limits are below the stipulated EU maximum residue limits for each of the penicillins.
本文介绍了一种使用液相色谱 - 电喷雾质谱法（LC - EMS）的确证分析方法，用于同时测定肌肉、肾脏和牛奶中的青霉素V、青霉素G以及苯唑西林、氯唑西林和双氯西林。萘夫西林用作内标。样品用乙腈提取，然后通过液 - 液萃取至二氯甲烷中进行净化。提取物注入专用的LC - EMS仪器，使用多离子单离子监测法检测存在的任何青霉素。每种青霉素可检测到三到四个离子，符合欧盟使用低分辨率质谱法进行确证残留分析的推荐标准之一。每种青霉素的检测限均低于欧盟规定的最大残留限量。
