Barden J A, Unsworth J
Physiol Chem Phys. 1975;7(1):31-8.
By observing diffraction spectra produced by passing a laser beam through stimulated muscle fibers, the magnitude of sarcomere length change and the relative rate of filament sliding were found to decrease in hypertonic solutions. The first order diffraction for both resting and stimulated muscle fibers faded and then returned to its original intensity as the bathing solution was changed from isotonic to hypertonic and then back to isotonic. This is explained as being due to a change in optical contrast between the A and I bands. 7 Hz oscillations were detected at 4 degrees C during stimulation as the fibers equilibrated in hypertonic solutions. The stiffness of resting muscle in the hypertonic solutions increased while the stiffness of stimulated muscle decreased until at a concentration of 3.10 R they were equal. All processes were reversible.
通过观察激光束穿过受刺激的肌纤维产生的衍射光谱，发现肌节长度变化的幅度和细丝滑动的相对速率在高渗溶液中会降低。当浴液从等渗变为高渗然后再变回等渗时，静息和受刺激肌纤维的一级衍射都会减弱，然后恢复到原来的强度。这被解释为是由于A带和I带之间光学对比度的变化所致。当纤维在高渗溶液中平衡时，在4摄氏度的刺激过程中检测到7赫兹的振荡。高渗溶液中静息肌肉的刚度增加，而受刺激肌肉的刚度降低，直到浓度为3.10R时它们相等。所有过程都是可逆的。
