Joseph B, Otta S K, Karunasagar I, Karunasagar I
Department of Fisher Microbiology College of Fisheries, Mangalore, India.
Int J Food Microbiol. 2001 Mar 20;64(3):367-72. doi: 10.1016/s0168-1605(00)00466-9.
Biofilm formation by two poultry isolates of Salmonella on three commonly used food contact surfaces viz plastic, cement and stainless steel were studied. Biofilm formation of both the isolates showed a similar trend with the highest density being on plastic followed by cement and steel. Salmonella weltevreden formed biofilm with a cell density of 3.4 x 10(7), 1.57 x 10(6) and 3 x 10(5) cfu/cm2 on plastic, cement and steel respectively while Salmonella FCM 40 biofilm on plastic, cement and steel were of the order of 1.2 x 10(7), 4.96 x 10(6) and 2.23 x 10(5) cfu/cm2 respectively. The sensitivity of the biofilm cells grown on these surfaces to different levels of two sanitizers namely hypochlorite and iodophor for varying exposure times was studied. Biofilm cells offered greater resistance when compared to their planktonic counterparts. Such biofilm cells in a food processing unit are not usually removed by the normal cleaning procedure and therefore could be a source of contamination of foods coming in contact with such surfaces.
研究了两种家禽源沙门氏菌在三种常用食品接触表面（即塑料、水泥和不锈钢）上的生物膜形成情况。两种分离株的生物膜形成呈现出相似的趋势，密度最高的是在塑料表面，其次是水泥表面和不锈钢表面。韦尔泰弗里登沙门氏菌在塑料、水泥和不锈钢上形成生物膜的细胞密度分别为3.4×10⁷、1.57×10⁶和3×10⁵ cfu/cm²，而FCM 40沙门氏菌在塑料、水泥和不锈钢上形成生物膜的细胞密度分别约为1.2×10⁷、4.96×10⁶和2.23×10⁵ cfu/cm²。研究了在这些表面上生长的生物膜细胞对两种消毒剂（即次氯酸盐和碘伏）不同浓度在不同暴露时间下的敏感性。与浮游菌相比，生物膜细胞具有更强的抗性。食品加工单元中的此类生物膜细胞通常不会通过正常清洁程序被去除，因此可能是与这些表面接触的食品的污染源。
