With 80% of bacterial infections occurring as biofilms, biofilm-related infections have evolved into a critical public health concern. Probiotics such as have emerged as promising alternatives, offering new avenues for effective treatment. This study aimed to evaluate the activity of against the growth, biofilm formation, motility, and quorum sensing (QS) of . Several experiments were conducted: The minimum inhibitory concentration (MIC) of against was determined to be 0.5 mg/mL using the broth microdilution method. The inhibition zone of 100 mg/mL of against was 19.98 ± 1.38 mm; the time-growth curve showed that can effectively inhibit the growth of . In biofilm experiments, at the MIC of , the inhibition rate of immature biofilm of was 86.9%, and it significantly reduced the production of biofilm components (EPS, e-DNA, and extracellular proteases) ( < 0.05). The disruption rate of mature biofilm by at the MIC was 66.89%, and it significantly decreased the levels of biofilm components (EPS and e-DNA) ( < 0.5). Microscopic observation showed that both the MIC and 1/2 MIC of could reduce the number of bacteria in the biofilm, which was not conducive to the formation and maintenance of the biofilm structure. Swimming/Swarming assays and QS experiments confirmed that inhibits the motility of and the secretion of AI-1-type quorum sensing molecules and downregulates the AI-2 quorum sensing system by upregulating gene expression. These findings suggest that could be a potential antimicrobial agent and biofilm inhibitor.