无乳支原体菌株PG2基因组的物理图谱。

A physical map of the Mycoplasma agalactiae strain PG2 genome.

作者信息

Tola S, Idini G, Rocchigiani A M, Rocca S, Manunta D, Leori G

机构信息

Istituto Zooprofilattico Sperimentale della Sardegna G. Pegreffi, 07100 Sassari, Italy.

出版信息

Vet Microbiol. 2001 May 21;80(2):121-30. doi: 10.1016/s0378-1135(00)00389-8.

DOI:10.1016/s0378-1135(00)00389-8

PMID:11295332

Abstract

We have constructed a physical map of the Mycoplasma agalactiae strain PG2 chromosome analyzing it by pulsed field gel electrophoresis in a contour-clamped homogeneous electric-field system. We mapped 33 cleavage sites generated with SmaI, XhoI, SalI, EclXI and BsiWI restriction endonucleases using double digestions, one- and two-dimensional pulsed electrophoresis, cross-hybridization and linking clones. We have also mapped the loci of some genes by Southern hybridization.

摘要

我们构建了无乳支原体菌株PG2染色体的物理图谱，通过在轮廓夹钳均匀电场系统中进行脉冲场凝胶电泳对其进行分析。我们使用双酶切、一维和二维脉冲电泳、交叉杂交和连接克隆，对由SmaI、XhoI、SalI、EclXI和BsiWI限制性内切酶产生的33个切割位点进行了定位。我们还通过Southern杂交对一些基因的位点进行了定位。