Miyata M, Wang L, Fukumura T
Department of Biology, Faculty of Science, Osaka City University, Japan.
FEMS Microbiol Lett. 1991 Apr 15;63(2-3):329-33. doi: 10.1016/0378-1097(91)90107-l.
A physical map of Mycoplasma capricolum ATCC 27343 genome was constructed, based on estimation of the restriction fragment sizes by pulse-field electrophoresis. The linkage order of restriction fragments was determined by two-dimensional electrophoresis of partial and complete single digests and complete double digests and by Southern hybridization analysis. The genome size was established at 1155.5 kb, and 26 cleavage sites for 7 endonucleases were assigned to the map.
基于脉冲场电泳对限制性片段大小的估计，构建了山羊支原体ATCC 27343基因组的物理图谱。通过部分和完全单酶切、完全双酶切的二维电泳以及Southern杂交分析确定了限制性片段的连锁顺序。基因组大小确定为1155.5 kb，并在图谱上标注了7种核酸内切酶的26个切割位点。
