830纳米照射可提高糖尿病小鼠模型的伤口抗张强度。

830-nm irradiation increases the wound tensile strength in a diabetic murine model.

作者信息

Stadler I, Lanzafame R J, Evans R, Narayan V, Dailey B, Buehner N, Naim J O

机构信息

The Laser Center, Rochester General Hospital, Rochester, New York 14621, USA.

出版信息

Lasers Surg Med. 2001;28(3):220-6. doi: 10.1002/lsm.1042.

DOI:10.1002/lsm.1042

PMID:11295756

Abstract

BACKGROUND AND OBJECTIVE

The purpose of this study was to investigate the effects of low-power laser irradiation on wound healing in genetic diabetes.

STUDY DESIGN/MATERIALS AND METHODS: Female C57BL/Ksj/db/db mice received 2 dorsal 1 cm full-thickness incisions and laser irradiation (830 nm, 79 mW/cm(2), 5.0 J/cm(2)/wound). Daily low-level laser therapy (LLLT) occurred over 0-4 days, 3-7 days, or nonirradiated. On sacrifice at 11 or 23 days, wounds were excised, and tensile strengths were measured and standardized.

RESULTS

Nontreated diabetic wound tensile strength was 0.77 +/- 0.22 g/mm(2) and 1.51 +/- 0.13 g/mm(2) at 11 and 23 days. After LLLT, over 0-4 days tensile strength was 1.15 +/- 0.14 g/mm(2) and 2.45 +/- 0.29 g/mm(2) (P = 0.0019). Higher tensile strength at 23 days occurred in the 3- to 7-day group (2.72 +/- 0.56 g/mm(2) LLLT vs. 1.51 +/- 0.13 g/mm(2) nontreated; P < or = 0.01).

CONCLUSION

Low-power laser irradiation at 830 nm significantly enhances cutaneous wound tensile strength in a murine diabetic model. Further investigation of the mechanism of LLLT in primary wound healing is warranted.

摘要

背景与目的

本研究旨在探讨低功率激光照射对遗传性糖尿病伤口愈合的影响。

研究设计/材料与方法：雌性C57BL/Ksj/db/db小鼠背部接受两处1厘米全层切口，并进行激光照射（830纳米，79毫瓦/平方厘米，5.0焦/平方厘米/伤口）。每日低强度激光治疗（LLLT）分别在0至4天、3至7天进行，或不进行照射。在第11天或第23天处死小鼠后，切除伤口，测量并标准化拉伸强度。

结果

未治疗的糖尿病伤口在第11天和第23天的拉伸强度分别为0.77±0.22克/平方毫米和1.51±0.13克/平方毫米。经过LLLT后，0至4天的拉伸强度为1.15±0.14克/平方毫米和2.45±0.29克/平方毫米（P = 0.0019）。在第23天，3至7天组的拉伸强度更高（LLLT组为2.72±0.56克/平方毫米，未治疗组为1.51±0.13克/平方毫米；P≤0.01）。