Stadler I, Lanzafame R J, Evans R, Narayan V, Dailey B, Buehner N, Naim J O
The Laser Center, Rochester General Hospital, Rochester, New York 14621, USA.
Lasers Surg Med. 2001;28(3):220-6. doi: 10.1002/lsm.1042.
The purpose of this study was to investigate the effects of low-power laser irradiation on wound healing in genetic diabetes.
STUDY DESIGN/MATERIALS AND METHODS: Female C57BL/Ksj/db/db mice received 2 dorsal 1 cm full-thickness incisions and laser irradiation (830 nm, 79 mW/cm(2), 5.0 J/cm(2)/wound). Daily low-level laser therapy (LLLT) occurred over 0-4 days, 3-7 days, or nonirradiated. On sacrifice at 11 or 23 days, wounds were excised, and tensile strengths were measured and standardized.
Nontreated diabetic wound tensile strength was 0.77 +/- 0.22 g/mm(2) and 1.51 +/- 0.13 g/mm(2) at 11 and 23 days. After LLLT, over 0-4 days tensile strength was 1.15 +/- 0.14 g/mm(2) and 2.45 +/- 0.29 g/mm(2) (P = 0.0019). Higher tensile strength at 23 days occurred in the 3- to 7-day group (2.72 +/- 0.56 g/mm(2) LLLT vs. 1.51 +/- 0.13 g/mm(2) nontreated; P < or = 0.01).
Low-power laser irradiation at 830 nm significantly enhances cutaneous wound tensile strength in a murine diabetic model. Further investigation of the mechanism of LLLT in primary wound healing is warranted.
本研究旨在探讨低功率激光照射对遗传性糖尿病伤口愈合的影响。
研究设计/材料与方法：雌性C57BL/Ksj/db/db小鼠背部接受两处1厘米全层切口，并进行激光照射（830纳米，79毫瓦/平方厘米，5.0焦/平方厘米/伤口）。每日低强度激光治疗（LLLT）分别在0至4天、3至7天进行，或不进行照射。在第11天或第23天处死小鼠后，切除伤口，测量并标准化拉伸强度。
未治疗的糖尿病伤口在第11天和第23天的拉伸强度分别为0.77±0.22克/平方毫米和1.51±0.13克/平方毫米。经过LLLT后，0至4天的拉伸强度为1.15±0.14克/平方毫米和2.45±0.29克/平方毫米（P = 0.0019）。在第23天，3至7天组的拉伸强度更高（LLLT组为2.72±0.56克/平方毫米，未治疗组为1.51±0.13克/平方毫米；P≤0.01）。
830纳米的低功率激光照射可显著提高小鼠糖尿病模型皮肤伤口的拉伸强度。有必要进一步研究LLLT在原发性伤口愈合中的作用机制。
