Norris D E, Shurtleff A C, Touré Y T, Lanzaro G C
The Johns Hopkins University, School of Hygiene and Public Health, The W Harry Feinstone Department of Molecular Microbiology and Immunology, Baltimore, MD 21205, USA.
J Med Entomol. 2001 Mar;38(2):336-40. doi: 10.1603/0022-2585-38.2.336.
We compared microsatellite polymorphism at nine loci located on chromosome 3 among two colonies and a field population of Anopheles gambiae sensu stricto Giles mosquitoes. Numbers of microsatellite alleles observed at each locus and mean heterozygosities were drastically reduced among laboratory colonies. Genetic analysis of the field population used in this study revealed an unprecedented frequency of rare alleles (<0.05). In contrast, colony samples revealed large numbers of alleles with frequencies >0.50. Partitioning of field data to assess the impact of rare alleles, null alleles, and sample size on estimates of mean heterozygosity revealed the plasticity of this measurement and suggests that heterozygosity may be reliably estimated from relatively small collections using microsatellites.
我们比较了冈比亚按蚊（Anopheles gambiae sensu stricto Giles）两个实验室菌落群体和一个野外种群中位于3号染色体上的9个位点的微卫星多态性。在每个位点观察到的微卫星等位基因数量和平均杂合度在实验室菌落群体中大幅降低。对本研究中使用的野外种群的遗传分析揭示了罕见等位基因（<0.05）的频率前所未有的高。相比之下，菌落样本显示大量等位基因的频率>0.50。对野外数据进行划分以评估罕见等位基因、无效等位基因和样本大小对平均杂合度估计的影响，揭示了该测量的可塑性，并表明使用微卫星从相对较小的样本中可以可靠地估计杂合度。
