González R H, Nusblat A, Nudel B C
Department of Industrial Microbiology and Biotechnology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
Microbiol Res. 2001 Mar;155(4):271-7. doi: 10.1016/S0944-5013(01)80004-5.
Quorum sensing is a widespread regulatory mechanism among Gram-negative bacteria. In this study, Acinetobacter strains were assayed for the presence of quorum sensing signal molecules capable of activating N-acylhomoserine lactone biosensors. By using an Agrobacterium tumefaciens reporter strain it was shown that all the cultures produced two to four detectable signal molecules with different chromatographic patterns. In A. calcoaceticus BD413 supernatants four compounds were detected in a time-dependent manner, and maximal activity was reached at stationary phase. The number of signal molecules was dependent on medium composition; typically, cultures in minimal medium displayed one or two more signals, as compared to complex medium. None of the Acinetobacter supematants showed autoinduction activity with an Chromobacterium violaceum reporter strain, neither in direct or competition assays.
群体感应是革兰氏阴性菌中一种广泛存在的调控机制。在本研究中，对不动杆菌菌株检测了是否存在能够激活N-酰基高丝氨酸内酯生物传感器的群体感应信号分子。通过使用根癌农杆菌报告菌株表明，所有培养物均产生了两到四种具有不同色谱模式的可检测信号分子。在醋酸钙不动杆菌BD413的上清液中，以时间依赖性方式检测到四种化合物，并且在稳定期达到最大活性。信号分子的数量取决于培养基组成；通常，与复杂培养基相比，基本培养基中的培养物显示出多一种或两种信号。在直接或竞争试验中，不动杆菌的任何上清液均未对紫色杆菌素报告菌株表现出自诱导活性。
