González R H, Dijkshoorn L, Van den Barselaar M, Nudel C
Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956 (1113) Ciudad Autónoma de Buenos Aires, Argentina.
Rev Argent Microbiol. 2009 Apr-Jun;41(2):73-8.
A set of 43 strains corresponding to 20 classified and unclassified genomic Acinetobacter species was analyzed for the production of typical N-acyl homoserine lactone quorum sensing molecules in culture broths. A large percentage of the strains (74%) displayed quorum sensing signals that could be separated into three statistically significantly different chromatographic groups (p < 0.001) based on their retention factor in TLC, i.e., Rf1 (0.22 +/- 0.02); Rf2 (0.40 +/- 0.02) and Rf3 (0.54 +/- 0.02). Noteworthy, 63% of the strains tested produced more than one quorum signal. The frequency of signal appearance was Rf3 > Rf2 > Rf1. None of the three signals could be specifically assigned to a particular species in the genus; furthermore, no distinction could be made between the quorum sensing signals secreted by typical opportunistic strains of the A. calcoaceticus-A. baumannii complex, isolated from patients, with respect to the other species of the genus, except for the Rf1 signal which was present in all the QS positive strains belonging to this complex and DNA group 13 TU. In conclusion, quorum sensors in Acinetobacter are not homogenously distributed among species and one of them is present in most of the A. calcoaceticus-baumannii complex.
对一组对应于20种已分类和未分类基因组不动杆菌属物种的43株菌株进行分析，以检测其在培养液中典型的N-酰基高丝氨酸内酯群体感应分子的产生情况。很大比例的菌株（74%）显示出群体感应信号，根据其在薄层层析（TLC）中的保留因子，这些信号可分为三个在统计学上有显著差异的色谱组（p < 0.001），即Rf1（0.22 +/- 0.02）；Rf2（0.40 +/- 0.02）和Rf3（0.54 +/- 0.02）。值得注意的是，63%的测试菌株产生了不止一种群体感应信号。信号出现的频率为Rf3 > Rf2 > Rf1。这三种信号均无法明确归属于该属中的某个特定物种；此外，从患者中分离出的乙酸钙不动杆菌-鲍曼不动杆菌复合体的典型机会性菌株所分泌的群体感应信号，与该属的其他物种之间没有区别，除了Rf1信号存在于属于该复合体和DNA组13 TU的所有群体感应阳性菌株中。总之，不动杆菌属中的群体感应传感器在物种间分布不均，其中一种存在于大多数乙酸钙不动杆菌-鲍曼不动杆菌复合体中。
