Agostini Hansjürgen T, Deckhut Alison, Jobes David V, Girones Rosina, Schlunck Günther, Prost Marcin G, Frias Carolina, Pérez-Trallero E, Ryschkewitsch Caroline F, Stoner Gerald L
Department of Ophthalmology, University of Freiburg, Killianstr. 5, 79106 Freiburg, Germany1.
Neurotoxicology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 36 Convent Drive, Room 4A-27, MD 20892-4126, Bethesda, USA2.
J Gen Virol. 2001 May;82(Pt 5):1221-1331. doi: 10.1099/0022-1317-82-5-1221.
Distinctive genotypes of JC virus have been described for the major continental landmasses. Studies on European-Americans and small cohorts in Europe showed predominantly Type 1. Types 2 and 7 are found in Asia, and Types 3 and 6 in Africa. These genotypes differ in sequence by about 1--3%. Each genotype may have several subtypes which differ from each other by about 0.5--1%. The genotypes can be defined by a distinctive pattern of nucleotides in a typing region of the VP1 gene. This genotyping approach has been confirmed by phylogenetic reconstruction using the entire genome exclusive of the rearranging regulatory region. In this first large European study, we report on the urinary excretion of JCV DNA of 350 individuals from Poland, Hungary, Germany and Spain. We included Gypsy cohorts in Hungary (Roma), Germany (Sinti), and Spain (Gitano), as well as Basques in Spain. We show that while Type 1 predominates in Europe, the proportions of Type 1A and 1B may differ from East to Southwest Europe. Type 4, closely related to the Type 1 sequence (only approximately 1% difference) was a minor genotype in Germany, Poland and Spain, but represented the majority in Basques. The Gitanos in Spain showed a variant Type 4 sequence termed 'Rom-1'. Interestingly, neither the Gitanos in Spain, nor Sinti or Roma in Germany or Hungary showed the Type 2 or Type 7 genotype that might be expected if their origins were in an Asian population.
已针对各大洲描述了JC病毒的不同基因型。对欧裔美国人及欧洲的小群体研究表明，主要为1型。2型和7型在亚洲发现，3型和6型在非洲发现。这些基因型在序列上相差约1%-3%。每个基因型可能有几个亚型，它们彼此之间相差约0.5%-1%。这些基因型可通过VP1基因分型区域中独特的核苷酸模式来定义。这种基因分型方法已通过使用不包括重排调控区域的全基因组进行系统发育重建得到证实。在这项首个大型欧洲研究中，我们报告了来自波兰、匈牙利、德国和西班牙的350人的JCV DNA尿排泄情况。我们纳入了匈牙利（罗姆人）、德国（辛提人）和西班牙（吉卜赛人）的吉卜赛群体，以及西班牙的巴斯克人。我们发现，虽然1型在欧洲占主导地位，但1A型和1B型的比例在欧洲东部和西南部可能有所不同。与1型序列密切相关（仅相差约1%）的4型在德国、波兰和西班牙是次要基因型，但在巴斯克人中占多数。西班牙的吉卜赛人显示出一种称为“Rom-1”的4型变异序列。有趣的是，西班牙的吉卜赛人、德国或匈牙利的辛提人或罗姆人都未显示出如果他们起源于亚洲人群可能预期的2型或7型基因型。
