Agostini H T, Ryschkewitsch C F, Stoner G L
Neurotoxicology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4126, USA.
J Gen Virol. 1998 Apr;79 ( Pt 4):801-5. doi: 10.1099/0022-1317-79-4-801.
The complete genomes of three new Type 1 strains of JC virus (JCV) from urine have been analysed. These were subtype 1A, subtype 1B and Type 4 as assigned from a short typing fragment in the VP1 gene. They differ from Mad1 (subtype 1A) by less than 1.0% of the DNA sequence. Based on its complete genome, the JCV Type 4 strain falls into a Type 1 subgroup. Type 4, with several Type 3-like sites in the short typing fragment, is a possible recombinant strain. The consensus of Type 1 DNA sequences is distinguished within the coding region from both Type 2 (strain GS/B) and five Type 3 (African and African American) strains at 64 sites. Most mutations are silent, but at 21 positions amino acid changes occur. Our findings define the subtypes of JCV Type 1 and support the validity of genotyping within the short VP1 fragment.
对来自尿液的三株新型1型JC病毒（JCV）的完整基因组进行了分析。根据VP1基因中的一个短分型片段，这些毒株分别为1A亚型、1B亚型和4型。它们与Mad1（1A亚型）的DNA序列差异小于1.0%。基于其完整基因组，JCV 4型毒株属于1型亚组。4型在短分型片段中有几个类似3型的位点，可能是重组毒株。1型DNA序列的共有序列在编码区内与2型（GS/B毒株）和五株3型（非洲和非裔美国人毒株）在64个位点存在差异。大多数突变是沉默突变，但在21个位置发生了氨基酸变化。我们的研究结果确定了JCV 1型的亚型，并支持在短VP1片段内进行基因分型的有效性。
