二酰基甘油对磷脂酰肌醇特异性磷脂酶C活性和囊泡融合的影响。
Diacylglycerol effects on phosphatidylinositol-specific phospholipase C activity and vesicle fusion.
作者信息
Villar A V, Goñi F M, Alonso A
机构信息
Unidad de Biofísica (CSIC-UPV/EHU), and Departamento de Bioquímica, Universidad del País Vasco, Aptdo. 644, 48080, Bilbao, Spain.
出版信息
FEBS Lett. 2001 Apr 6;494(1-2):117-20. doi: 10.1016/s0014-5793(01)02333-x.
Diacylglycerol increased the hydrolytic activity of phosphatidylinositol-specific phospholipase C on large unilamellar vesicles containing 5-40% phosphatidylinositol. Moreover, diacylglycerol increased the rate and extent of vesicle fusion (contents mixing) induced by the enzyme. Kinetic studies of intervesicular lipid mixing revealed that fusion was limited by the frequency of contacts involving two diacylglycerol-rich domains.
二酰基甘油增加了磷脂酰肌醇特异性磷脂酶C对含有5 - 40%磷脂酰肌醇的大单层囊泡的水解活性。此外，二酰基甘油增加了该酶诱导的囊泡融合（内容物混合）的速率和程度。囊泡间脂质混合的动力学研究表明，融合受涉及两个富含二酰基甘油结构域的接触频率限制。
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