Ikawati Z, Wahyuono S, Maeyama K
Department of Pharmacology Ehime University School of Medicine, Shigenobu-cho, Ehime 791-0295, Onsen-gun, Japan.
J Ethnopharmacol. 2001 May;75(2-3):249-56. doi: 10.1016/s0378-8741(01)00201-x.
Twelve alcoholic extracts and 12 hexane extracts of plant materials selected on the basis of medicinal folklore for asthma treatment in Indonesia were studied for their activity in inhibiting histamine release from RBL-2H3 cells (rat basophilic leukemia cell line), a tumor analog of mast cells. The results of screening indicated that five alcoholic extracts (Plantago major leaves, Eucalyptus globulus leaves and fruit, Cinnamomum massoiae cortex, Vitex trifolia leaves) and two hexane extracts (Eucalyptus globulus leaves, Vitex trifolia leaves) inhibited IgE-dependent histamine release from RBL-2H3 cells. The inhibitory effects were found to be more than 80% for extract concentrations of 0.5 mg/ml. The results indicate that the extracts contain active compounds that inhibit mast-cell degranulation, and provide insight into the development of new drugs for treating asthma and/or allergic disease.
在印度尼西亚，根据治疗哮喘的民间医学经验挑选了一些植物材料，对其12种乙醇提取物和12种己烷提取物进行了研究，以考察它们抑制RBL-2H3细胞（大鼠嗜碱性白血病细胞系，肥大细胞的肿瘤类似物）释放组胺的活性。筛选结果表明，5种乙醇提取物（大车前叶、蓝桉叶和果实、爪哇桂皮、三叶牡荆叶）和2种己烷提取物（蓝桉叶、三叶牡荆叶）可抑制RBL-2H3细胞中IgE依赖性组胺释放。提取物浓度为0.5 mg/ml时，抑制率超过80%。结果表明，这些提取物含有抑制肥大细胞脱颗粒的活性化合物，为开发治疗哮喘和/或过敏性疾病的新药提供了思路。
