车前草药用植物不同提取物的抗增殖特性、生物相容性和化学成分。
Anti-Proliferative Properties, Biocompatibility, and Chemical Composition of Different Extracts of Plantago major Medicinal Plant.
机构信息
Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan; Iran.
出版信息
Iran Biomed J. 2021 Mar 1;25(2):106-16. doi: 10.29252/ibj.25.2.106.
BACKGROUND
To study the anticancer activity of Plantago major, we assessed the effect of ethanolic, methanolic and acetonic extracts of this plant on HCT-116, SW-480, and HEK-293 cell lines as control.
METHODS
The cytotoxic activity, biocompatibility, and toxicity were evaluated by MTT assay, hemolysis, and Artemia salina-LD50 (on mice) tests, respectively. The analysis of the extracts was performed by GC-MS analysis.
RESULTS
The results showed that all the extracts had the most antiproliferative properties on the HCT-116 cell line. The P. major root extract was more effective than the aerial parts, and IC50 values for ethanolic, methanolic and acetonic root extracts were 405.59, 470.16, and 82.26 µg/mL, respectively on HCT-116 cell line at 72 h. Hemolysis degree of the ethanolic extract of aerial and root parts were approximately 1% at 400 μg/mL.. Using the ethanolic extracts, the Artemia survived every concentration, and no toxicity was observed. One week after the oral administration of different parts of P. major extracts, none of the mice died, even those were administered 2000 mg/kg. The results of GC/MS analysis showed that P. major extracts contain potential anticancer compounds, such as stearic acid (8.61%) in aerial parts of methanolic extract and 1,2- Benzenedicarboxylic acid, mono(2-ethylhexyl)ester (88.07% and 40.63%) in aerial and root parts of acetonic extract of P. major.
CONCLUSION
Our findings suggest that the P. major is a source of potential compounds with antiproliferative properties.
背景
为了研究车前草的抗癌活性，我们评估了该植物的乙醇、甲醇和丙酮提取物对 HCT-116、SW-480 和 HEK-293 细胞系的影响作为对照。
方法
通过 MTT 测定、溶血和卤虫致死试验（在小鼠上）分别评估细胞毒性、生物相容性和毒性。通过 GC-MS 分析对提取物进行分析。
结果
结果表明，所有提取物对 HCT-116 细胞系均具有最强的增殖抑制作用。车前草根提取物比地上部分更有效，乙醇、甲醇和丙酮根提取物对 HCT-116 细胞系的 IC50 值分别为 405.59、470.16 和 82.26 µg/mL，在 72 小时。400 µg/mL 时，地上和根部分的乙醇提取物的溶血程度约为 1%。使用乙醇提取物，卤虫在每一种浓度下都存活下来，没有观察到毒性。在口服不同部分的车前草提取物一周后，即使给予 2000 mg/kg，也没有老鼠死亡。GC/MS 分析结果表明，车前草提取物含有潜在的抗癌化合物，如甲醇提取物中地上部分的硬脂酸（8.61%）和丙酮提取物中地上和根部分的 1,2-苯二甲酸、单（2-乙基己基）酯（88.07% 和 40.63%）。
结论
我们的研究结果表明，车前草是具有增殖抑制特性的潜在化合物的来源。
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