Tayebi H, Kuttler F, Saas P, Lienard A, Petracca B, Lapierre V, Ferrand C, Fest T, Cahn J, Blaise D, Kuentz M, Hervé P, Tiberghien P, Robinet E
Etablissement Français du Sang Bourgogne/Franche-Comté, 1, Bd Alexandre Fleming, 25020 Besançon cedex, France.
Exp Hematol. 2001 Apr;29(4):458-70. doi: 10.1016/s0301-472x(01)00613-0.
Some phenotypic and functional properties of lymphocytes from bone marrow or peripheral blood stem cell donors were compared in a randomized study. Lymphocyte subsets were analyzed by immunocytometry in blood harvested from bone marrow donors (n = 27) and from peripheral blood stem cell donors before and after granulocyte colony-stimulating factor mobilization (n = 23) and in bone marrow and peripheral blood stem cell grafts. Granulocyte colony-stimulating factor mobilization increased the blood T and B, but not NK, lymphocyte counts. All lymphocyte counts were approximately 10-fold higher in peripheral blood stem cell grafts than in bone marrow grafts. Analysis of CD25, CD95, HLA-DR, and CD45RA expression shows that T-cell activation level was lower after granulocyte colony-stimulating factor mobilization. Similarly, granulocyte colony-stimulating factor reduced by twofold to threefold the percentage of interferon-gamma, interleukin-2, and tumor necrosis factor-alpha-secreting cells within the NK, NK-T, and T-cell subsets and severely impaired the potential for interferon-gamma production at the single-cell level. mRNA levels of both type 1 (interferon-gamma, interleukin-2) and type 2 (interleukin-4, interleukin-13) cytokines were approximately 10-fold lower in peripheral blood stem cell grafts than in bone marrow grafts. This reduced potential of cytokine production was not associated with a preferential mobilization of so-called "suppressive" cells (CD3+CD4-CD8-, CD3+CD8+CD56+, or CD3+TCRVA24+CD161+), nor with a modulation of killer cell receptors CD161, NKB1, and CD94 expression by NK, NK-T, or T cells. Our data demonstrate in a randomized setting that quantitative as well as qualitative differences exist between a bone marrow and a peripheral blood stem cell graft, whose ability to produce type 1 and type 2 cytokines is impaired.
在一项随机研究中，对来自骨髓或外周血干细胞供者的淋巴细胞的一些表型和功能特性进行了比较。通过免疫细胞计数法分析了从骨髓供者（n = 27）以及外周血干细胞供者在粒细胞集落刺激因子动员前后采集的血液（n = 23）中的淋巴细胞亚群，以及骨髓和外周血干细胞移植物中的淋巴细胞亚群。粒细胞集落刺激因子动员增加了血液中T淋巴细胞和B淋巴细胞的数量，但未增加自然杀伤（NK）细胞的数量。外周血干细胞移植物中的所有淋巴细胞数量比骨髓移植物中的大约高10倍。对CD25、CD95、HLA-DR和CD45RA表达的分析表明，粒细胞集落刺激因子动员后T细胞活化水平较低。同样，粒细胞集落刺激因子使NK细胞、NK-T细胞和T细胞亚群中分泌干扰素-γ、白细胞介素-2和肿瘤坏死因子-α的细胞百分比降低了两倍至三倍，并在单细胞水平上严重损害了干扰素-γ的产生潜力。1型（干扰素-γ、白细胞介素-2）和2型（白细胞介素-4、白细胞介素-13）细胞因子的mRNA水平在外周血干细胞移植物中比在骨髓移植物中低约10倍。这种细胞因子产生潜力的降低与所谓“抑制性”细胞（CD3 + CD4 - CD8 -、CD3 + CD8 + CD56 +或CD3 + TCRVA24 + CD161 +）的优先动员无关，也与NK细胞、NK-T细胞或T细胞对杀伤细胞受体CD161、NKB1和CD94表达的调节无关。我们的数据在随机研究中表明，骨髓移植物和外周血干细胞移植物之间存在数量和质量上的差异，其产生1型和2型细胞因子的能力受损。
